Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 47353..47996 Replicon plasmid pSE11-3
Accession NC_011416
Organism Escherichia coli SE11

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag ECSE_RS25880 Protein ID WP_001044768.1
Coordinates 47353..47769 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag ECSE_RS25885 Protein ID WP_001261287.1
Coordinates 47766..47996 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECSE_RS25855 42794..43891 - 1098 WP_012565218.1 choloylglycine hydrolase family protein -
ECSE_RS28135 43934..44152 - 219 WP_001292878.1 hypothetical protein -
ECSE_RS25860 44301..45110 + 810 Protein_47 (S)-acetoin forming diacetyl reductase -
ECSE_RS28985 45482..45643 - 162 WP_001384894.1 hypothetical protein -
ECSE_RS28140 45716..47191 + 1476 Protein_49 transposase -
ECSE_RS25880 47353..47769 - 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
ECSE_RS25885 47766..47996 - 231 WP_001261287.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
ECSE_RS25890 48181..48623 - 443 Protein_52 transposase -
ECSE_RS25905 49207..50091 - 885 WP_000942222.1 helix-turn-helix domain-containing protein -
ECSE_RS28155 50376..51206 + 831 WP_012565223.1 helix-turn-helix domain-containing protein -
ECSE_RS25915 51251..52000 - 750 WP_106141716.1 EAL domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - faeC / faeD / faeE / faeF / faeG / faeH / faeI / faeJ 1..60555 60555
- inside IScluster/Tn - - 45716..48623 2907


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T23224 WP_001044768.1 NC_011416:c47769-47353 [Escherichia coli SE11]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T23224 NC_011416:c47769-47353 [Escherichia coli SE11]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGTATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGAGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT23224 WP_001261287.1 NC_011416:c47996-47766 [Escherichia coli SE11]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT23224 NC_011416:c47996-47766 [Escherichia coli SE11]
ATGAGAACCGTATCGATATTTAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References