Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 48197..48840 Replicon plasmid unnamed1
Accession NZ_CP090963
Organism Edwardsiella piscicida strain CZ12001

Toxin (Protein)


Gene name vagD Uniprot ID Q84A06
Locus tag L1P06_RS17835 Protein ID WP_000754566.1
Coordinates 48424..48840 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag L1P06_RS17830 Protein ID WP_001261276.1
Coordinates 48197..48427 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L1P06_RS17790 (L1P06_17790) 43380..43607 - 228 WP_236893505.1 hypothetical protein -
L1P06_RS17795 (L1P06_17795) 43706..44344 - 639 WP_236893506.1 ParA family protein -
L1P06_RS17800 (L1P06_17800) 44728..44967 + 240 WP_236893508.1 hypothetical protein -
L1P06_RS17805 (L1P06_17805) 45018..45380 + 363 WP_236893510.1 hypothetical protein -
L1P06_RS17810 (L1P06_17810) 45456..46265 - 810 WP_236893432.1 site-specific integrase -
L1P06_RS17815 (L1P06_17815) 46262..46885 - 624 WP_236893434.1 DUF2913 family protein -
L1P06_RS17820 (L1P06_17820) 47008..47652 - 645 WP_236893436.1 hypothetical protein -
L1P06_RS17825 (L1P06_17825) 47787..48141 - 355 Protein_56 hypothetical protein -
L1P06_RS17830 (L1P06_17830) 48197..48427 + 231 WP_001261276.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
L1P06_RS17835 (L1P06_17835) 48424..48840 + 417 WP_000754566.1 type II toxin-antitoxin system VapC family toxin Toxin
L1P06_RS17840 (L1P06_17840) 48921..49625 + 705 WP_001067858.1 IS6-like element IS26 family transposase -
L1P06_RS17845 (L1P06_17845) 49678..49809 - 132 Protein_60 SMR family transporter -
L1P06_RS17850 (L1P06_17850) 49926..50764 - 839 Protein_61 AadA family aminoglycoside 3''-O-nucleotidyltransferase -
L1P06_RS17855 (L1P06_17855) 50770..51060 - 291 WP_001336345.1 DUF1010 domain-containing protein -
L1P06_RS17860 (L1P06_17860) 51172..51669 - 498 WP_001083725.1 trimethoprim-resistant dihydrofolate reductase DfrA12 -
L1P06_RS17865 (L1P06_17865) 51814..52827 + 1014 WP_000845039.1 class 1 integron integrase IntI1 -
L1P06_RS17870 (L1P06_17870) 53030..53380 + 351 WP_000454193.1 DUF3330 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aadA2 / dfrA12 / tet(A) / qnrS1 - 1..90212 90212


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15006.52 Da        Isoelectric Point: 9.2957

>T232235 WP_000754566.1 NZ_CP090963:48424-48840 [Edwardsiella piscicida]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Download         Length: 417 bp

>T232235 NZ_CP127274:c3055204-3054809 [Mycobacterium tuberculosis]
GTGATCGTCGATACGTCGGCCATCGTCGCCATCGTGAGCGGGGAATCGGGCGCGCAGGTGCTCAAGGAGGCGCTGGAGCG
GTCACCGAACTCCCGAATGTCCGCGCCCAACTACGTCGAACTGTGCGCGATCATGCAGCGGCGGGACCGGCCGGAGATCT
CTCGATTGGTGGACCGTTTGCTGGACGACTACGGAATCCAGGTCGAAGCCGTCGACGCCGACCAAGCCCGCGTGGCCGCG
CAGGCGTATCGCGACTACGGCCGCGGCAGCGGCCATCCGGCCCGTCTCAACCTCGGCGACACCTACAGCTACGCCCTGGC
CCAGGTCACCGGGGAGCCGCTGCTGTTTCGTGGCGATGACTTCACGCATACCGATATCCGGCCCGCGTGCACCTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT232235 WP_001261276.1 NZ_CP090963:48197-48427 [Edwardsiella piscicida]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT232235 NZ_CP127274:c3054783-3054517 [Mycobacterium tuberculosis]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A387K1G3

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References