Detailed information of TA system TA232222

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rlegTA/AbiEii(toxin)
• Location: 2973571..2974987
• Replicon: chromosome
• Accession: NZ_CP090959
• Organism: Simiduia sp. 21SJ11W-1

Toxin (Protein)

• Gene name: rlegT
• Uniprot ID: -
• Locus tag: L1F30_RS12990
• Protein ID: WP_253356662.1
• Coordinates: 2973571..2974473 (-)
• Length: 301 a.a.

Antitoxin (Protein)

• Gene name: rlegA
• Uniprot ID: -
• Locus tag: L1F30_RS12995
• Protein ID: WP_253361884.1
• Coordinates: 2974544..2974987 (-)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L1F30_RS12975 (L1F30_12970)	2969384..2970580	+	1197	WP_253356659.1	hypothetical protein	-
L1F30_RS12980 (L1F30_12975)	2970583..2971455	-	873	WP_253356660.1	glutathione-dependent disulfide-bond oxidoreductase	-
L1F30_RS12985 (L1F30_12980)	2971710..2973299	+	1590	WP_253356661.1	ABC-F family ATPase	-
L1F30_RS12990 (L1F30_12985)	2973571..2974473	-	903	WP_253356662.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
L1F30_RS12995 (L1F30_12990)	2974544..2974987	-	444	WP_253361884.1	transcriptional regulator	Antitoxin
L1F30_RS13000 (L1F30_13000)	2975425..2975943	-	519	WP_253356663.1	hypothetical protein	-
L1F30_RS13005 (L1F30_13005)	2976239..2976607	-	369	WP_253356664.1	transcriptional regulator	-
L1F30_RS13010 (L1F30_13010)	2976641..2976943	-	303	WP_253356665.1	type II toxin-antitoxin system HigB family toxin	-
L1F30_RS13015 (L1F30_13015)	2977072..2977308	+	237	WP_253361833.1	helix-turn-helix domain-containing protein	-
L1F30_RS13020 (L1F30_13020)	2977580..2978101	+	522	WP_253356666.1	DUF2778 domain-containing protein	-
L1F30_RS13025 (L1F30_13025)	2978082..2978462	+	381	WP_253356667.1	DUF2195 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (37-270)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 301 a.a.
• Molecular weight: 34401.25 Da
• Isoelectric Point: 5.7759

>T232222 WP_253356662.1 NZ_CP090959:c2974473-2973571 [Simiduia sp. 21SJ11W-1]
MNTTKNIAASVRQKLLNRARSDKRPFAELLQYYAMERFLYRLSQSTHSKHFILKGALMLRVWESPEFRPTMDIDMLGKTS
NDEANIVSQITEILNLAVEPDGLDFDVDSIQSERIKEDADYQGVRIRFRGTLDSARINMQIDIGFGDIVHPCPEECDLPT
MLDFTAPRLLCYSRESAIAEKFEAMVKLGVLNSRMKDFYDIWLLSRQFNFNGSQLAQAIQLTFKQRGTALSDNIVAFQAP
FIEEKQVQWAAFCRRINQEHLPNDFSDIVAQVTSFLAPVIEAARSGQPFVKSWTAANEWR

Nucleotide

• Length: 903 bp

>T232222 NZ_CP127274:c2218583-2218239 [Mycobacterium tuberculosis]
GTGGTGATTAGTCGTGCCGAGATCTACTGGGCTGACCTCGGGCCGCCATCAGGCAGTCAGCCGGCGAAGCGCCGCCCGGT
GCTCGTAATCCAGTCAGATCCGTACAACGCAAGTCGCCTTGCCACTGTGATCGCAGCGGTGATCACGTCCAATACGGCGC
TGGCGGCAATGCCCAGCAACGTGTTCTTGCCCGCGACCACAACGCGACTGCCACGTGACTCGGTCGTCAACGTCACGGCG
ATTGTCACGCTCAACAAGACTGACCTCACCGACCGAGTTGGGGAGGTGCCAGCGAGCTTGATGCACGAGGTTGACCGAGG
ACTTCGTCGCGTACTGGACCTTTGA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16617.50 Da
• Isoelectric Point: 7.7215

>AT232222 WP_253361884.1 NZ_CP090959:c2974987-2974544 [Simiduia sp. 21SJ11W-1]
MDQDQISDPDLVIVAKRVPQAVICLVSALAYHDITTQIPHAVSIALPKGAETPRVDYPPISVHRFSEAALKEGVETHQID
GVSINIYCPEKTLADCFKFRNKLGMDIVLEALKFYKARKTFNLEKLIMYARVCRVEKIMRPYLEATL

Nucleotide

• Length: 444 bp

>AT232222 NZ_CP127274:c2218825-2218577 [Mycobacterium tuberculosis]
ATGAAGACAGCTATTTCTCTGCCGGATGAGACGTTCGATCGGGTATCGCGGCGTGCGAGTGAGCTCGGCATGAGTCGGTC
CGAGTTCTTCACGAAGGCTGCGCAGCGCTACCTGCACGAGCTGGACGCCCAATTGCTCACGGGCCAGATCGACAGGGCTC
TAGAGAGCATCCATGGCACCGACGAAGCGGAGGCCCTCGCCGTGGCCAACGCATACCGCGTGCTAGAAACCATGGACGAT
GAGTGGTGA