Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 19809..20452 Replicon plasmid p1194_11_4
Accession NZ_CP090935
Organism Klebsiella pneumoniae strain ONE259

Toxin (Protein)


Gene name vagD Uniprot ID R4IT02
Locus tag I7858_RS28000 Protein ID WP_016338372.1
Coordinates 20036..20452 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID R4IT22
Locus tag I7858_RS27995 Protein ID WP_016338373.1
Coordinates 19809..20039 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I7858_RS27965 (I7858_27965) 15406..16272 - 867 WP_004118283.1 replication initiation protein -
I7858_RS27970 (I7858_27970) 16807..16911 + 105 WP_032409716.1 hypothetical protein -
I7858_RS27975 (I7858_27975) 17040..17297 + 258 WP_000764642.1 hypothetical protein -
I7858_RS27980 (I7858_27980) 17355..18131 - 777 WP_000015958.1 site-specific integrase -
I7858_RS27985 (I7858_27985) 18128..18871 - 744 WP_000129823.1 hypothetical protein -
I7858_RS27990 (I7858_27990) 18922..19272 - 351 WP_000493378.1 hypothetical protein -
I7858_RS27995 (I7858_27995) 19809..20039 + 231 WP_016338373.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
I7858_RS28000 (I7858_28000) 20036..20452 + 417 WP_016338372.1 type II toxin-antitoxin system VapC family toxin Toxin
I7858_RS28005 (I7858_28005) 20493..20816 - 324 WP_085842301.1 hypothetical protein -
I7858_RS28010 (I7858_28010) 20834..21370 - 537 WP_223825639.1 hypothetical protein -
I7858_RS28015 (I7858_28015) 22029..23306 + 1278 WP_016338369.1 HlyD family secretion protein -
I7858_RS28020 (I7858_28020) 23296..25404 + 2109 WP_016338368.1 peptidase domain-containing ABC transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaCTX-M-15 / aac(3)-IId - 1..34269 34269


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-50)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15060.57 Da        Isoelectric Point: 9.2738

>T232209 WP_016338372.1 NZ_CP090935:20036-20452 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHIELVDAFCARLDAILPWDRA
AVDATTEIKVALRQAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFARVPGLVLEDWVK

Download         Length: 417 bp

>T232209 NZ_CP127274:1239609-1239983 [Mycobacterium tuberculosis]
GTGATCCTGGTCGACACTTCGGTATGGATTGAGCACCTGCGCGCCGCCGACGCGCGACTCGTCGAGCTGCTGGGCGATGA
CGAGGCCGGTTGCCATCCGCTCGTCATCGAGGAGCTGGCGCTTGGCTCGATCAAGCAGCGAGACGTTGTTCTCGATCTGT
TGGCCAACCTCTACCAGTTTCCGGTGGTGACCCACGACGAAGTGTTGCGGCTTGTCGGTCGGCGGCGGTTGTGGGGTCGG
GGACTCGGTGCCGTCGATGCCAACCTTCTTGGTTCGGTGGCTCTGGTTGGCGGCGCGCGACTATGGACGCGGGACAAGCG
GTTGAAGGCGGCGTGCGCGGAAAGCGGTGTTGCGCTGGCTGAGGAAGTGTCCTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8751.77 Da        Isoelectric Point: 4.3533

>AT232209 WP_016338373.1 NZ_CP090935:19809-20039 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWSSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT232209 NZ_CP127274:1239415-1239612 [Mycobacterium tuberculosis]
ATGAGGACGACGGTGACCGTTGACGACGCCTTGTTAGCCAAAGCGGCCGAATTGACTGGGGTGAAAGAGAAGTCGACGCT
CCTGCGCGAGGGGTTGCAGACACTGGTCCGGGTGGAGAGCGCCCGGCGGTTGGCGGCTCTCGGCGGCACCGACCCGCAAG
CTACCGCGGCGCCGAGACGCCGGACGTCGCCCCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB R4IT02

Antitoxin

Source ID Structure
AlphaFold DB A0A5Q9LN61

References