Detailed information of TA system TA232208

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/GNAT-DUF1778
• Location: 227719..228470
• Replicon: plasmid p1194_11_1
• Accession: NZ_CP090932
• Organism: Klebsiella pneumoniae strain ONE259

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: A0A8T3UHS1
• Locus tag: I7858_RS27010
• Protein ID: WP_004883482.1
• Coordinates: 227719..228201 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: A0A8T3UKU4
• Locus tag: I7858_RS27015
• Protein ID: WP_004883484.1
• Coordinates: 228192..228470 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I7858_RS26995 (I7858_26995)	223545..225278	+	1734	WP_025368656.1	PP2C family serine/threonine-protein phosphatase	-
I7858_RS27000 (I7858_27000)	225306..226805	+	1500	WP_004196935.1	lipopolysaccharide kinase InaA family protein	-
I7858_RS27005 (I7858_27005)	227184..227666	+	483	WP_004196929.1	hypothetical protein	-
I7858_RS27010 (I7858_27010)	227719..228201	-	483	WP_004883482.1	GNAT family N-acetyltransferase	Toxin
I7858_RS27015 (I7858_27015)	228192..228470	-	279	WP_004883484.1	DUF1778 domain-containing protein	Antitoxin
I7858_RS27020 (I7858_27020)	228986..229954	-	969	WP_234552789.1	IS5 family transposase	-
I7858_RS27025 (I7858_27025)	230759..231034	-	276	WP_001141269.1	formaldehyde-responsive transcriptional repressor FrmR	-
I7858_RS28545	232735..233109	-	375	WP_227522231.1	DUF488 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaTEM-1A / blaOXA-9 / ant(3'')-Ia / aac(6')-Ib / sul1 / qacE / aadA2 / dfrA15 / ARR-3 / dfrA8 / rmtG / sul2 / catA1 / formA	-	1..266289	266289

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (69-145)

Antitoxin

• Vibrio phage ICP1, Orf50 (10-86)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17491.36 Da
• Isoelectric Point: 9.4950

>T232208 WP_004883482.1 NZ_CP090932:c228201-227719 [Klebsiella pneumoniae]
MGMRPPEPLTPEHNIAEFCCQDQVINEWLKKKALRNHGTGISRVFVVCSENSKRVIAYYCLSSGSVHRNTVTGTYRRNAP
AAIPVIVLGRLAVDAAWAGKGIGAALLKDAIYRTEHIAVQVGVRALLVHALNDEVVAFYTKFGFETSAVNALTLLFPVKV

Nucleotide

• Length: 483 bp

>T232208 NZ_CP127274:c1230970-1230659 [Mycobacterium tuberculosis]
ATGCGACCTATCCACATCGCACAGCTTGACAAGGCTAGACCCGTCCTGATCCTTACCCGCGAGGTCGTACGTCCGCACCT
CACGAACGTCACCGTCGCCCCGATCACGACAACCGTGCGTGGGCTAGCCACCGAAGTCCCGGTAGACGCGGTGAACGGGC
TGAATCAACCGTCAGTCGTCAGCTGCGACAACACCCAAACGATCCCGGTGTGTGACCTTGGTCGTCAAATCGGCTACCTG
CTCGCGTCGCAGGAGCCGGCTCTAGCCGAGGCCATCGGCAACGCGTTCGACCTCGATTGGGTGGTGGCATAG

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10720.23 Da
• Isoelectric Point: 4.5423

>AT232208 WP_004883484.1 NZ_CP090932:c228470-228192 [Klebsiella pneumoniae]
MKPDVREAPINIRAKTSQRDLIDMAANLVSKSRTDFMLEAACREAQDILLDQRLFLLDDKQFDTFIEELDAPVTPERQAR
IDNLMNRKSPWE

Nucleotide

• Length: 279 bp

>AT232208 NZ_CP127274:c1231290-1230970 [Mycobacterium tuberculosis]
ATGTACCTACCCTGGGGGGTCGTCCTCGCCGGCGGCGCGAACGGTTTTGGGGCGGGTGCATACCAAACTGGTACCATCTG
TGAAGTGAGTACACAGATTGCGGTTCGCTTGCCCGATGAGATTGTCGCGTTCATCGACGACGAAGTCCGGGGACAGCATG
CCCGTAGCCGTGCGGCTGTCGTGCTGCGGGCGCTGGAACGGGAACGCCGCCGTCGTCTCGCCGAACGCGACGCGGAGATC
CTCGCCACCAACACGTCAGCCACGGGTGATCTGGATACGCTGGCCGGCCACTGCGCTCGTACCGCCCTCGACATCGACTG
A