Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 137237..138147 Replicon plasmid p1194_11_1
Accession NZ_CP090932
Organism Klebsiella pneumoniae strain ONE259

Toxin (Protein)


Gene name - Uniprot ID A0A2N4Z2R0
Locus tag I7858_RS26550 Protein ID WP_004197882.1
Coordinates 137677..138147 (+) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A2J4RLY9
Locus tag I7858_RS26545 Protein ID WP_004181895.1
Coordinates 137237..137680 (+) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I7858_RS26520 (I7858_26520) 133028..133450 + 423 WP_004197884.1 translesion error-prone DNA polymerase V autoproteolytic subunit -
I7858_RS26525 (I7858_26525) 133450..133953 + 504 Protein_133 DNA polymerase V subunit UmuC -
I7858_RS26530 (I7858_26530) 134030..134794 - 765 WP_004184534.1 IS21-like element helper ATPase IstB -
I7858_RS26535 (I7858_26535) 134791..136290 - 1500 WP_004184532.1 IS21-like element ISPlge2 family transposase -
I7858_RS26540 (I7858_26540) 136387..137136 + 750 Protein_136 DUF4113 domain-containing protein -
I7858_RS26545 (I7858_26545) 137237..137680 + 444 WP_004181895.1 DUF2384 domain-containing protein Antitoxin
I7858_RS26550 (I7858_26550) 137677..138147 + 471 WP_004197882.1 RES family NAD+ phosphorylase Toxin
I7858_RS26555 (I7858_26555) 138257..138517 - 261 WP_004026352.1 hypothetical protein -
I7858_RS26560 (I7858_26560) 139207..140222 + 1016 Protein_140 IS3-like element ISKpn8 family transposase -
I7858_RS26570 (I7858_26570) 140991..141113 + 123 Protein_142 IS3 family transposase -
I7858_RS26575 (I7858_26575) 141291..142631 + 1341 WP_004196353.1 IS1202-like element ISEsa1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaTEM-1A / blaOXA-9 / ant(3'')-Ia / aac(6')-Ib / sul1 / qacE / aadA2 / dfrA15 / ARR-3 / dfrA8 / rmtG / sul2 / catA1 / formA - 1..266289 266289
- inside IScluster/Tn - - 129054..140615 11561


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17633.92 Da        Isoelectric Point: 4.5152

>T232206 WP_004197882.1 NZ_CP090932:137677-138147 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDFFTLLSIDVPDELIQSANMD
ELPDNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T232206 NZ_CP127274:1014865-1015299 [Mycobacterium tuberculosis]
ATGGCGAAACTGTCCGGATCCATCGACGTACCGCTGCCACCGGAGGAAGCCTGGATGCACGCCTCCGATCTGACTCGTTA
CCGAGAGTGGCTGACCATCCACAAGGTATGGCGCAGCAAGTTGCCCGAAGTGCTCGAGAAGGGCACGGTCGTCGAGTCGT
ATGTCGAGGTCAAGGGCATGCCCAACCGGATCAAGTGGACGATCGTGCGGTACAAACCCCCGGAGGGCATGACGCTCAAC
GGCGACGGTGTGGGTGGTGTCAAAGTCAAGCTGATCGCTAAGGTAGCGCCGAAAGAGCACGGCTCCGTCGTCAGCTTCGA
TGTGCACCTCGGCGGCCCGGCCCTGCTCGGGCCGATCGGCATGATCGTCGCCGCTGCATTGCGAGCCGACATCCGCGAAT
CGCTGCAGAACTTCGTCACGGTGTTTGCCGGCTGA

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16536.87 Da        Isoelectric Point: 10.3013

>AT232206 WP_004181895.1 NZ_CP090932:137237-137680 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT232206 NZ_CP127274:1014680-1014859 [Mycobacterium tuberculosis]
ATGGGAATCCTGGACAAGGTAAAGAACCTGCTGTCGCAGAACGCCGACAAGGTCGAGACGGTGATCAACAAAGCGGGCGA
ATTCGTCGACGAGCAGACGCAAGGCAATTATTCTGACGCCATCCACAAGCTGCATGACGCGGCCAGCAACGTCGTCGGCA
TGAGCGACCAGCAGAGCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2N4Z2R0

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4RLY9

References