Detailed information of TA system TA232205

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): stbDE/ParE-DnaT
• Location: 44543..45065
• Replicon: plasmid p1194_11_1
• Accession: NZ_CP090932
• Organism: Klebsiella pneumoniae strain ONE259

Toxin (Protein)

• Gene name: stbE
• Uniprot ID: A0A2J4R0S6
• Locus tag: I7858_RS26105
• Protein ID: WP_004181778.1
• Coordinates: 44781..45065 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: stbD
• Uniprot ID: A0A5Q9LM53
• Locus tag: I7858_RS26100
• Protein ID: WP_025368601.1
• Coordinates: 44543..44791 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I7858_RS26080 (I7858_26080)	39752..40573	-	822	WP_004026535.1	hypothetical protein	-
I7858_RS26085 (I7858_26085)	40635..40988	-	354	WP_004181774.1	hypothetical protein	-
I7858_RS26090 (I7858_26090)	41133..42119	-	987	WP_025368599.1	hypothetical protein	-
I7858_RS26095 (I7858_26095)	42453..44252	-	1800	WP_025368600.1	ATP-dependent helicase	-
I7858_RS26100 (I7858_26100)	44543..44791	+	249	WP_025368601.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
I7858_RS26105 (I7858_26105)	44781..45065	+	285	WP_004181778.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I7858_RS26110 (I7858_26110)	45295..46521	+	1227	WP_025368602.1	RNA-guided endonuclease TnpB family protein	-
I7858_RS26115 (I7858_26115)	46807..47232	-	426	WP_040120489.1	IS200/IS605 family transposase	-
I7858_RS26120 (I7858_26120)	47269..48495	+	1227	WP_025368604.1	RNA-guided endonuclease TnpB family protein	-
I7858_RS26125 (I7858_26125)	48561..49687	-	1127	Protein_52	conjugal transfer protein TraG N-terminal domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaTEM-1A / blaOXA-9 / ant(3'')-Ia / aac(6')-Ib / sul1 / qacE / aadA2 / dfrA15 / ARR-3 / dfrA8 / rmtG / sul2 / catA1 / formA	-	1..266289	266289

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-84)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10970.69 Da
• Isoelectric Point: 10.6516

>T232205 WP_004181778.1 NZ_CP090932:44781-45065 [Klebsiella pneumoniae]
MTYKLAFNESALKEWKKLGHTIREQFKKKLAERLLNPRVPASQLHGRKDQYKIKLRGAGYRLVYSVNDDVVTVTVIGVGK
RENDDIYNLTKHRN

Nucleotide

• Length: 285 bp

>T232205 NZ_CP127274:841227-841655 [Mycobacterium tuberculosis]
ATGTTCCTTCTCGACGCCAACGTGCTGCTGGCTGCACACCGCGGTGACCACCCGAATCACCGAACCGTCCGCCCCTGGTT
CGATCGACTGCTCGCGGCTGACGACCCCTTCACAGTGCCGAACCTGGTATGGGCGTCGTTCCTCCGGCTGGCAACGAATC
GACGCATCTTCGAGATTCCGTCACCGCGAGCAGAGGCATTCGCATTCGTCGAAGCCGTCACCGCCCAGCCCCATCACCTT
CCGACGAACCCCGGTCCCAGACACCTCATGCTGCTGCGAAAACTCTGCGACGAGGCCGACGCATCGGGCGACTTGATACC
TGACGCGGTACTCGCGGCCATAGCAGTGGGGCATCACTGCGCCGTGGTGAGCCTGGACAGGGATTTCGCCCGGTTTGCCT
CGGTGCGCCACATTCGCCCGCCGCTCTAG

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8906.12 Da
• Isoelectric Point: 3.8206

>AT232205 WP_025368601.1 NZ_CP090932:44543-44791 [Klebsiella pneumoniae]
MAFQVLTTTAASITELKRDPMGTFNAGEGAPVAILNRNEPAFYCVPPALYAQLMELLEDQELGHIADERIGEAVIEVSLD
DL

Nucleotide

• Length: 249 bp

>AT232205 NZ_CP127274:840946-841203 [Mycobacterium tuberculosis]
ATGCGCACCACGGTGTCAATCTCCGATGAAATACTCGCTGCCGCCAAACGCCGGGCCCGCGAGCGTGGTCAATCGCTGGG
CGCTGTGATCGAGGACGCCCTTCGGCGGGAGTTCGCCGCCGCCCACGTCGGCGGCGCCCGCCCGACCGTCCCGGTTTTCG
ACGGCGGCACCGGTCCGCGGCGAGGCATCGACCTGACCTCGAATAGAGCGTTGTCCGAAGTGCTCGACGAGGGCCTGGAA
CTGAACTCCCGGAAGTAA

Structures

Toxin

• AlphaFold DB: A0A2J4R0S6

Antitoxin

• AlphaFold DB: A0A5Q9LM53