Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5206237..5206862 Replicon chromosome
Accession NZ_CP090931
Organism Klebsiella pneumoniae strain ONE259

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag I7858_RS25500 Protein ID WP_002882817.1
Coordinates 5206237..5206620 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag I7858_RS25505 Protein ID WP_004150355.1
Coordinates 5206620..5206862 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I7858_RS25485 (I7858_25485) 5203603..5204505 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
I7858_RS25490 (I7858_25490) 5204502..5205137 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
I7858_RS25495 (I7858_25495) 5205134..5206063 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
I7858_RS25500 (I7858_25500) 5206237..5206620 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
I7858_RS25505 (I7858_25505) 5206620..5206862 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
I7858_RS25510 (I7858_25510) 5207067..5207984 + 918 WP_002882812.1 alpha/beta hydrolase -
I7858_RS25515 (I7858_25515) 5207998..5208939 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
I7858_RS25520 (I7858_25520) 5208984..5209421 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
I7858_RS25525 (I7858_25525) 5209418..5210278 - 861 WP_002882807.1 virulence factor BrkB family protein -
I7858_RS25530 (I7858_25530) 5210272..5210871 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T232204 WP_002882817.1 NZ_CP090931:c5206620-5206237 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T232204 NZ_CP127274:c755771-755334 [Mycobacterium tuberculosis]
ATGATAGTGCTCGACACCACCGTCCTCGTCTATGCCAAAGGCGCCGAGCATCCACTCCGCGATCCCTGCCGCGATCTCGT
CGCGGCAATCGCCGACGAACGCATCGCTGCGACCACCACCGCCGAGGTGATCCAGGAGTTCGTCCACGTACGCGCCCGCC
GGCGCGACCGCAGCGACGCCGCCGCGCTGGGCCGTGTGACTATGCCGAACTGCTCTCGCCGCTACTCACCATCAATCGAG
GCCACCTCGAAGCGCGGCCTGACGCTGTTCGAGACAACGCCAGGGCTTGAAGCCTGCGACGCGGTGCTCGCGGCGGTTGC
CGCAAGCGCCGGCGCGACCGCTCTTGTCTCGGCTGACCCGGCGTTCGCCGATCTGTCGGATGTCGTGCACGTCATTCCCG
ACGCGGCCGGGATGGTCAGCCTGCTCGGCGACCGCTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT232204 WP_004150355.1 NZ_CP090931:c5206862-5206620 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT232204 NZ_CP127274:c756022-755768 [Mycobacterium tuberculosis]
ATGAGCATGCGTCTGGCGCATCGGCTCCAGATCCTGCTCGACGACGAGTGCCACCGCCGCATCACCGCGGTGGCGCGCGA
GCGAGGCGTCCCGGTCGCAACCGTCGTGCGTGAGGCCATTGATCGAGGCCTCGTCAGCCCCGCCGGTCGCCGCAAGTCCG
CGGGCAGGCGCCTGCTCGACGCCGCCGACATGTCCGTTCCCGAGCCGCGGGAGCTGAAGCAGGAACTCGAGGCCCTCCGG
GCGCGCCGCGGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References