23220

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 3870021..3870652 Replicon chromosome
Accession NC_011415
Organism Escherichia coli SE11

Toxin (Protein)


Gene name hicA Uniprot ID F4NNK6
Locus tag ECSE_RS19475 Protein ID WP_001259388.1
Coordinates 3870377..3870652 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag ECSE_RS19470 Protein ID WP_000593555.1
Coordinates 3870021..3870380 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECSE_RS19445 3866153..3867097 + 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
ECSE_RS19450 3867094..3867927 + 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
ECSE_RS19455 3867927..3868691 + 765 WP_001136236.1 nickel import ATP-binding protein NikD -
ECSE_RS19460 3868688..3869494 + 807 WP_000173666.1 nickel import ATP-binding protein NikE -
ECSE_RS19465 3869500..3869901 + 402 WP_001190064.1 nickel-responsive transcriptional regulator NikR -
ECSE_RS19470 3870021..3870380 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
ECSE_RS19475 3870377..3870652 - 276 WP_001259388.1 type II toxin-antitoxin system HicA family toxin Toxin
ECSE_RS19480 3870725..3871849 - 1125 WP_001615305.1 ABC transporter permease -
ECSE_RS19485 3871849..3874584 - 2736 WP_000149132.1 ribosome-associated ATPase/putative transporter RbbA -
ECSE_RS19490 3874581..3875648 - 1068 WP_000361482.1 HlyD family efflux transporter periplasmic adaptor subunit -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10151.89 Da        Isoelectric Point: 10.7076

>T23220 WP_001259388.1 NC_011415:c3870652-3870377 [Escherichia coli SE11]
MRTKVQALRKKQKNTLDQIVKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T23220 NC_011415:c3870652-3870377 [Escherichia coli SE11]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATAGTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGCAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT23220 WP_000593555.1 NC_011415:c3870380-3870021 [Escherichia coli SE11]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT23220 NC_011415:c3870380-3870021 [Escherichia coli SE11]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FFA2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References