Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3285962..3286616 Replicon chromosome
Accession NC_011415
Organism Escherichia coli SE11

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag ECSE_RS16460 Protein ID WP_000244772.1
Coordinates 3285962..3286369 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag ECSE_RS16465 Protein ID WP_000354046.1
Coordinates 3286350..3286616 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECSE_RS16440 3281919..3283652 - 1734 WP_000813220.1 single-stranded-DNA-specific exonuclease RecJ -
ECSE_RS16445 3283658..3284368 - 711 WP_000715208.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
ECSE_RS16450 3284393..3285289 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
ECSE_RS16455 3285401..3285922 + 522 WP_001055874.1 flavodoxin FldB -
ECSE_RS16460 3285962..3286369 - 408 WP_000244772.1 protein YgfX Toxin
ECSE_RS16465 3286350..3286616 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
ECSE_RS16470 3286859..3287839 + 981 WP_000886095.1 tRNA-modifying protein YgfZ -
ECSE_RS16475 3288035..3288694 - 660 WP_000250274.1 hemolysin III family protein -
ECSE_RS16480 3288858..3289169 - 312 WP_001182945.1 N(4)-acetylcytidine aminohydrolase -
ECSE_RS16485 3289214..3290647 + 1434 WP_001307385.1 6-phospho-beta-glucosidase BglA -
ECSE_RS16490 3290704..3291447 - 744 WP_000951941.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T23218 WP_000244772.1 NC_011415:c3286369-3285962 [Escherichia coli SE11]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T23218 NC_011415:c3286369-3285962 [Escherichia coli SE11]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT23218 WP_000354046.1 NC_011415:c3286616-3286350 [Escherichia coli SE11]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT23218 NC_011415:c3286616-3286350 [Escherichia coli SE11]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References