Detailed information of TA system TA232136

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/MazF-doc
• Location: 26482..27065
• Replicon: plasmid pSE45A5-2
• Accession: NZ_CP090917
• Organism: Staphylococcus epidermidis strain 45A5

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: KB229_RS12495
• Protein ID: WP_234966670.1
• Coordinates: 26482..26820 (-)
• Length: 113 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: KB229_RS12500
• Protein ID: WP_095325081.1
• Coordinates: 26817..27065 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KB229_RS12455 (KB229_12455)	21883..22038	+	156	WP_234966666.1	hypothetical protein	-
KB229_RS12460 (KB229_12460)	22190..22369	+	180	WP_203084990.1	hypothetical protein	-
KB229_RS12465 (KB229_12465)	22332..23089	+	758	Protein_20	IS3 family transposase	-
KB229_RS12470 (KB229_12470)	23176..23460	-	285	WP_234966667.1	hypothetical protein	-
KB229_RS12475 (KB229_12475)	23503..24105	-	603	WP_234966668.1	hypothetical protein	-
KB229_RS12480 (KB229_12480)	24424..24519	+	96	WP_019236174.1	type I toxin-antitoxin system Fst family toxin	-
KB229_RS12485 (KB229_12485)	24695..25615	+	921	WP_234966669.1	HEPN/Toprim-associated domain-containing protein	-
KB229_RS12490 (KB229_12490)	25686..26360	-	675	WP_234966623.1	IS6 family transposase	-
KB229_RS12495 (KB229_12495)	26482..26820	-	339	WP_234966670.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KB229_RS12500 (KB229_12500)	26817..27065	-	249	WP_095325081.1	AbrB family transcriptional regulator	Antitoxin
KB229_RS12505 (KB229_12505)	27642..27974	+	333	WP_234966671.1	hypothetical protein	-
KB229_RS12510 (KB229_12510)	28111..28332	+	222	WP_234966672.1	hypothetical protein	-
KB229_RS12515 (KB229_12515)	28400..28882	+	483	WP_234966673.1	hypothetical protein	-
KB229_RS12520 (KB229_12520)	29240..30421	+	1182	WP_234966674.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..30442	30442

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-106)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 113 a.a.
• Molecular weight: 13065.28 Da
• Isoelectric Point: 10.0482

>T232136 WP_234966670.1 NZ_CP090917:c26820-26482 [Staphylococcus epidermidis]
MSIKQFDIYYIDLDPTRGREKRKVRPCLIVNNQMTINGTNFVWILPITSREVKFPTDIKVKTKNGLVTGVIDTIQIRALD
INARYHNYKDELQENLKSDVLKAIQTYLKPIL

Nucleotide

• Length: 339 bp

>T232136 NZ_CP127273:c694539-694147 [Mycobacterium tuberculosis]
GTGAACGTACGGCGCGCCCTCGCCGACACATCCGTTTTCATTGGCATCGAGGCGACACGATTCGACCCGGACCGGTTTGC
GGGATACGAATGGGGCGTCTCGGTAGTCACACTCGGCGAGCTGCGGCTCGGGGTGTTGCAAGCCTCCGGCCCCGAGGCCG
CCGCACGTCGGCTCTCCACCTACCAGCTTGCGCAACGGTTCGAACCACTAGGCATCGACGAGGCGGTCTCCGAAGCATGG
GCATTACTGGTGTCCAAGCTGCGCGCCGCCAAACTGCGCGTGCCGATCAACGACAGCTGGATTGCGGCCACAGCCGTGGC
GCACGGCATTGCGATCCTGACCCAAGACAACGACTACGCCGCCATGCCCGACGTCGAGGTCATAACGATCTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9176.23 Da
• Isoelectric Point: 4.4944

>AT232136 WP_095325081.1 NZ_CP090917:c27065-26817 [Staphylococcus epidermidis]
MNKTSERIFKSGNSKAISLGKQTIQLTDFEVGDKVEVQQVDGGLFITKKEQSIEDEITNFFKNGGKYTEPEIDFGESVGR
EI

Nucleotide

• Length: 249 bp

>AT232136 NZ_CP127273:c694793-694536 [Mycobacterium tuberculosis]
ATGTCTGCTACGATACCCGCTCGCGATCTTCGCAACCACACCGCCGAGGTACTGCGGCGAGTTGCCGCCGGCGAGGAAAT
CGAGGTGCTCAAGGACAATCGCCCCGTAGCGCGCATCGTTCCGCTCAAGCGGCGCCGCCAATGGTTGCCAGCTGCCGAGG
TGATCGGCGAACTGGTGCGCTTGGGCCCCGATACCACCAATCTGGGCGAGGAGCTGCGAGAGACGCTGACGCAAACCACG
GACGATGTGCGGTGGTGA