Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 62504..63141 Replicon plasmid pM1
Accession NZ_CP090910
Organism Enterobacter hormaechei strain M1

Toxin (Protein)


Gene name vagD Uniprot ID A0A9E6WXZ4
Locus tag L1E13_RS22440 Protein ID WP_032662169.1
Coordinates 62504..62914 (-) Length 137 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID A0A9E6WTF5
Locus tag L1E13_RS22445 Protein ID WP_032662167.1
Coordinates 62911..63141 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L1E13_RS22420 (L1E13_22420) 57870..58220 + 351 WP_032662182.1 DUF305 domain-containing protein -
L1E13_RS22425 (L1E13_22425) 58406..59314 - 909 WP_032662179.1 HNH endonuclease -
L1E13_RS22430 (L1E13_22430) 59861..60883 - 1023 WP_032662174.1 helicase UvrD -
L1E13_RS22435 (L1E13_22435) 60868..62430 - 1563 WP_032662171.1 AAA family ATPase -
L1E13_RS22440 (L1E13_22440) 62504..62914 - 411 WP_032662169.1 PIN domain-containing protein Toxin
L1E13_RS22445 (L1E13_22445) 62911..63141 - 231 WP_032662167.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
L1E13_RS22450 (L1E13_22450) 63683..63973 + 291 WP_013087126.1 hypothetical protein -
L1E13_RS22455 (L1E13_22455) 64104..64634 + 531 WP_013087127.1 hypothetical protein -
L1E13_RS22460 (L1E13_22460) 64653..65429 + 777 WP_015572054.1 site-specific integrase -
L1E13_RS22465 (L1E13_22465) 65631..65888 + 258 Protein_67 site-specific integrase -
L1E13_RS22470 (L1E13_22470) 66132..67142 - 1011 WP_015572055.1 RepB family plasmid replication initiator protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid catA2 / tet(D) / dfrA14 / sul2 / aph(3'')-Ib / aph(6)-Id / qnrS1 / blaLAP-2 - 1..148434 148434


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-127)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 137 a.a.        Molecular weight: 14729.03 Da        Isoelectric Point: 5.7351

>T232123 WP_032662169.1 NZ_CP090910:c62914-62504 [Enterobacter hormaechei]
VNKMYMLDTNICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITWAEVSLASQEAGPAAQKLADAFAARLDAILPWDRTAV
DATTEIKVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVR

Download         Length: 411 bp

>T232123 NZ_CP127255:4925937-4926314 [Escherichia coli]
ATGAAAACATTGCCTGTATTACCCGGGCAGGCGGCCGGTTCACGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTTAC
CAGACTGCTGGACCAGCATTACGGCCTCACGCTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGAACCGACCAGCCGGGGTTC
AGCACCTGTACCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGGCCCGCGA
CAATTACAGAACGGTAAATAACATTACCTTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8645.77 Da        Isoelectric Point: 4.4692

>AT232123 WP_032662167.1 NZ_CP090910:c63141-62911 [Enterobacter hormaechei]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMAERGDVVSDEGRVNL

Download         Length: 231 bp

>AT232123 NZ_CP127255:4925479-4925847 [Escherichia coli]
GTGTCAGACACACTCCATGAAACAAATTATCCCGACGACAACAACGACCGCTCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTCAGGGCACGTCTGGTGCAGGAAGGGAACCGGTTGCATTACCTTGCAGACCGTGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTCAGTCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References