Detailed information of TA system TA232119

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 3043029..3043625
• Replicon: chromosome
• Accession: NZ_CP090909
• Organism: Enterobacter hormaechei strain M1

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A837F9W8
• Locus tag: L1E13_RS14440
• Protein ID: WP_023315213.1
• Coordinates: 3043323..3043625 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: L1E13_RS14435
• Protein ID: WP_023315212.1
• Coordinates: 3043029..3043316 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L1E13_RS14430 (L1E13_14430)	3041401..3043032	+	1632	WP_003860350.1	Na/Pi cotransporter family protein	-
L1E13_RS14435 (L1E13_14435)	3043029..3043316	-	288	WP_023315212.1	putative addiction module antidote protein	Antitoxin
L1E13_RS14440 (L1E13_14440)	3043323..3043625	-	303	WP_023315213.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
L1E13_RS14445 (L1E13_14445)	3043823..3044695	+	873	WP_045346556.1	23S rRNA pseudouridine(2604) synthase RluF	-
L1E13_RS14450 (L1E13_14450)	3044696..3044968	-	273	WP_003860346.1	DUF3811 domain-containing protein	-
L1E13_RS14455 (L1E13_14455)	3045019..3045963	-	945	WP_234978774.1	ketopantoate/pantoate/pantothenate transporter PanS	-
L1E13_RS14460 (L1E13_14460)	3046057..3047406	-	1350	WP_045895713.1	lysine-sensitive aspartokinase 3	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-93)

Antitoxin

• Uncharacterized protein HI1420 (3-90)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11427.21 Da
• Isoelectric Point: 10.1042

>T232119 WP_023315213.1 NZ_CP090909:c3043625-3043323 [Enterobacter hormaechei]
MKEIVQTESFRRWEQNLKDRRAKTIIASRLFRLANGLAGDIRPVGEGISELRIHFGPGYRVYFKDQGNCIIVLLCGGDKS
SQARDILMAKMLSNVSQWQE

Nucleotide

• Length: 303 bp

>T232119 NZ_CP127255:c3845095-3844844 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10171.73 Da
• Isoelectric Point: 7.0086

>AT232119 WP_023315212.1 NZ_CP090909:c3043316-3043029 [Enterobacter hormaechei]
MHKLTPYDPANALVDDEEIAVFMADALETGDSAYIAKALGVIARAKGMSTISLQTGLSREQLYRSFSDKGNPTLKTTLAV
MKALGLRLTIKPSGD

Nucleotide

• Length: 288 bp

>AT232119 NZ_CP127255:c3844847-3844593 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Toxin

• AlphaFold DB: A0A837F9W8