Detailed information of TA system TA232085

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 84206..84972
• Replicon: chromosome
• Accession: NZ_CP090908
• Organism: Serratia marcescens strain Sys06

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: A0A0P0Q8U6
• Locus tag: L1F17_RS00395
• Protein ID: WP_060558096.1
• Coordinates: 84604..84972 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: L1F17_RS00390
• Protein ID: WP_076606738.1
• Coordinates: 84206..84541 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L1F17_RS00360 (L1F17_00360)	79554..79775	-	222	WP_060558082.1	AlpA family transcriptional regulator	-
L1F17_RS00365 (L1F17_00365)	80238..81095	+	858	WP_060558089.1	hypothetical protein	-
L1F17_RS00370 (L1F17_00370)	81192..82082	+	891	WP_076606737.1	50S ribosome-binding GTPase	-
L1F17_RS00375 (L1F17_00375)	82330..82884	+	555	WP_060558091.1	hypothetical protein	-
L1F17_RS00380 (L1F17_00380)	82912..83532	+	621	WP_060560398.1	hypothetical protein	-
L1F17_RS00385 (L1F17_00385)	83714..84193	+	480	WP_060558094.1	DNA repair protein RadC	-
L1F17_RS00390 (L1F17_00390)	84206..84541	+	336	WP_076606738.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
L1F17_RS00395 (L1F17_00395)	84604..84972	+	369	WP_060558096.1	TA system toxin CbtA family protein	Toxin
L1F17_RS00400 (L1F17_00400)	85201..86055	+	855	WP_060560399.1	DUF4942 domain-containing protein	-
L1F17_RS00405 (L1F17_00405)	86355..86546	+	192	WP_015376193.1	host cell division inhibitor Icd-like protein	-
L1F17_RS00410 (L1F17_00410)	86543..86806	+	264	WP_033639063.1	hypothetical protein	-
L1F17_RS00415 (L1F17_00415)	86803..87138	+	336	WP_033631431.1	DUF5375 family protein	-
L1F17_RS00420 (L1F17_00420)	87149..89497	+	2349	WP_060558099.1	primase-helicase zinc-binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (7-106)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13663.64 Da
• Isoelectric Point: 6.7085

>T232085 WP_060558096.1 NZ_CP090908:84604-84972 [Serratia marcescens]
MQTLPVTPKRAAYACPSPVVVWQSMLTYLLEQHYGLALNDTEFSDDTVIHEHIDAGISLADALNFTVEKFGLVRTDRRGF
SCQEQSPFITAIDILRARRATGLMTRMGYQTITSVIRGEKQA

Nucleotide

• Length: 369 bp

>T232085 NZ_CP127252:c1530594-1530316 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12479.13 Da
• Isoelectric Point: 6.9656

>AT232085 WP_076606738.1 NZ_CP090908:84206-84541 [Serratia marcescens]
MSSKTTPAWGLKRNVTPQFGARLVQEGDRLHFLADRADISGKFSDEQVRDLNNAFPQFINHLELMLLSGELNPRYQHCVT
LYHKGLICEADSLGSHGYVYLAIYPTPQSTA

Nucleotide

• Length: 336 bp

>AT232085 NZ_CP127252:c1530857-1530597 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Toxin

• AlphaFold DB: A0A0P0Q8U6