Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) spoIISABC/SpoIISA-SpoIISB
Location 1238612..1239529 Replicon chromosome
Accession NZ_CP090905
Organism Bacillus velezensis strain Yao

Toxin (Protein)


Gene name spoIISA Uniprot ID A0A6A8LGT8
Locus tag L0961_RS06510 Protein ID WP_003154806.1
Coordinates 1238783..1239529 (-) Length 249 a.a.

Antitoxin (Protein)


Gene name spoIISB Uniprot ID I2HQ14
Locus tag L0961_RS06505 Protein ID WP_003154807.1
Coordinates 1238612..1238782 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L0961_RS06465 (L0961_06465) 1233843..1235465 + 1623 WP_003154821.1 pyocin knob domain-containing protein -
L0961_RS06470 (L0961_06470) 1235478..1235849 + 372 WP_014304856.1 XkdW family protein -
L0961_RS06475 (L0961_06475) 1235855..1236052 + 198 WP_003154819.1 XkdX family protein -
L0961_RS06480 (L0961_06480) 1236109..1236870 + 762 WP_003154817.1 hypothetical protein -
L0961_RS06485 (L0961_06485) 1236922..1237185 + 264 WP_003154815.1 hemolysin XhlA family protein -
L0961_RS06490 (L0961_06490) 1237199..1237462 + 264 WP_003154813.1 phage holin -
L0961_RS06495 (L0961_06495) 1237476..1238354 + 879 WP_014304858.1 N-acetylmuramoyl-L-alanine amidase -
L0961_RS06500 (L0961_06500) 1238390..1238515 - 126 WP_003154809.1 hypothetical protein -
L0961_RS06505 (L0961_06505) 1238612..1238782 - 171 WP_003154807.1 type II toxin-antitoxin system SpoIISB family antitoxin Antitoxin
L0961_RS06510 (L0961_06510) 1238783..1239529 - 747 WP_003154806.1 type II toxin-antitoxin system SpoIISA family toxin Toxin
L0961_RS06515 (L0961_06515) 1239634..1240632 - 999 WP_003154805.1 inorganic phosphate transporter -
L0961_RS06520 (L0961_06520) 1240645..1241262 - 618 WP_003154804.1 DUF47 domain-containing protein -
L0961_RS06525 (L0961_06525) 1241548..1242864 - 1317 WP_046559522.1 amino acid permease -
L0961_RS06530 (L0961_06530) 1243188..1244138 + 951 WP_069013314.1 ring-cleaving dioxygenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(3-239)

Antitoxin

Antitoxin SpoIISB

(1-56)


Sequences

Toxin        


Download         Length: 249 a.a.        Molecular weight: 29034.50 Da        Isoelectric Point: 4.6947

>T232082 WP_003154806.1 NZ_CP090905:c1239529-1238783 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVVGAMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYGEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQDRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Download         Length: 747 bp

>T232082 NZ_CP127252:649362-649673 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6676.74 Da        Isoelectric Point: 10.9273

>AT232082 WP_003154807.1 NZ_CP090905:c1238782-1238612 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Download         Length: 171 bp

>AT232082 NZ_CP127252:649674-649964 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTATAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6A8LGT8

Antitoxin

Source ID Structure
AlphaFold DB I2HQ14

References