Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | mazEF/MazF(toxin) |
| Location | 472676..473313 | Replicon | chromosome |
| Accession | NZ_CP090905 | ||
| Organism | Bacillus velezensis strain Yao | ||
Toxin (Protein)
| Gene name | mazF | Uniprot ID | G4NU33 |
| Locus tag | L0961_RS02420 | Protein ID | WP_003156187.1 |
| Coordinates | 472963..473313 (+) | Length | 117 a.a. |
Antitoxin (Protein)
| Gene name | mazE | Uniprot ID | I2HMS5 |
| Locus tag | L0961_RS02415 | Protein ID | WP_003156188.1 |
| Coordinates | 472676..472957 (+) | Length | 94 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| L0961_RS02395 (L0961_02395) | 469041..469640 | - | 600 | WP_015388676.1 | rhomboid family intramembrane serine protease | - |
| L0961_RS02400 (L0961_02400) | 469733..470098 | + | 366 | WP_003156192.1 | holo-ACP synthase | - |
| L0961_RS02405 (L0961_02405) | 470263..471270 | + | 1008 | WP_003156191.1 | outer membrane lipoprotein carrier protein LolA | - |
| L0961_RS02410 (L0961_02410) | 471387..472556 | + | 1170 | WP_003156189.1 | alanine racemase | - |
| L0961_RS02415 (L0961_02415) | 472676..472957 | + | 282 | WP_003156188.1 | type II toxin-antitoxin system antitoxin EndoAI | Antitoxin |
| L0961_RS02420 (L0961_02420) | 472963..473313 | + | 351 | WP_003156187.1 | type II toxin-antitoxin system endoribonuclease NdoA | Toxin |
| L0961_RS02425 (L0961_02425) | 473431..474252 | + | 822 | WP_003156182.1 | STAS domain-containing protein | - |
| L0961_RS02430 (L0961_02430) | 474257..474622 | + | 366 | WP_003156180.1 | RsbT antagonist protein RsbS | - |
| L0961_RS02435 (L0961_02435) | 474625..475026 | + | 402 | WP_003156178.1 | anti-sigma regulatory factor | - |
| L0961_RS02440 (L0961_02440) | 475038..476045 | + | 1008 | WP_003156177.1 | PP2C family protein-serine/threonine phosphatase | - |
| L0961_RS02445 (L0961_02445) | 476109..476438 | + | 330 | WP_003156176.1 | anti-sigma factor antagonist RsbV | - |
| L0961_RS02450 (L0961_02450) | 476435..476917 | + | 483 | WP_003156173.1 | anti-sigma B factor RsbW | - |
| L0961_RS02455 (L0961_02455) | 476883..477671 | + | 789 | WP_003156171.1 | RNA polymerase sigma factor SigB | - |
| L0961_RS02460 (L0961_02460) | 477671..478273 | + | 603 | WP_234949564.1 | PP2C family serine/threonine-protein phosphatase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 117 a.a. Molecular weight: 12977.98 Da Isoelectric Point: 4.8781
>T232081 WP_003156187.1 NZ_CP090905:472963-473313 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF
Download Length: 351 bp
>T232081 NZ_CP127252:c186627-186397 [Escherichia coli]
CTGGATCAGCTATTTAAATCACCTTTACCTCAAGGAATCAAATGGACTGATATAGAATCTCTGGTTAAAGCGTTAGGCGG
AGAAATTAAGGAAGGTAGGGGGTCGCGCTGTAAGTTCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGT
CGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTGCGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA
CTGGATCAGCTATTTAAATCACCTTTACCTCAAGGAATCAAATGGACTGATATAGAATCTCTGGTTAAAGCGTTAGGCGG
AGAAATTAAGGAAGGTAGGGGGTCGCGCTGTAAGTTCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGT
CGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTGCGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA
Antitoxin
Download Length: 94 a.a. Molecular weight: 10562.92 Da Isoelectric Point: 6.4706
>AT232081 WP_003156188.1 NZ_CP090905:472676-472957 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG
Download Length: 282 bp
>AT232081 NZ_CP127252:c186400-186041 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|