Detailed information of TA system TA232081

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 472676..473313
• Replicon: chromosome
• Accession: NZ_CP090905
• Organism: Bacillus velezensis strain Yao

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: L0961_RS02420
• Protein ID: WP_003156187.1
• Coordinates: 472963..473313 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: L0961_RS02415
• Protein ID: WP_003156188.1
• Coordinates: 472676..472957 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0961_RS02395 (L0961_02395)	469041..469640	-	600	WP_015388676.1	rhomboid family intramembrane serine protease	-
L0961_RS02400 (L0961_02400)	469733..470098	+	366	WP_003156192.1	holo-ACP synthase	-
L0961_RS02405 (L0961_02405)	470263..471270	+	1008	WP_003156191.1	outer membrane lipoprotein carrier protein LolA	-
L0961_RS02410 (L0961_02410)	471387..472556	+	1170	WP_003156189.1	alanine racemase	-
L0961_RS02415 (L0961_02415)	472676..472957	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
L0961_RS02420 (L0961_02420)	472963..473313	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
L0961_RS02425 (L0961_02425)	473431..474252	+	822	WP_003156182.1	STAS domain-containing protein	-
L0961_RS02430 (L0961_02430)	474257..474622	+	366	WP_003156180.1	RsbT antagonist protein RsbS	-
L0961_RS02435 (L0961_02435)	474625..475026	+	402	WP_003156178.1	anti-sigma regulatory factor	-
L0961_RS02440 (L0961_02440)	475038..476045	+	1008	WP_003156177.1	PP2C family protein-serine/threonine phosphatase	-
L0961_RS02445 (L0961_02445)	476109..476438	+	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
L0961_RS02450 (L0961_02450)	476435..476917	+	483	WP_003156173.1	anti-sigma B factor RsbW	-
L0961_RS02455 (L0961_02455)	476883..477671	+	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
L0961_RS02460 (L0961_02460)	477671..478273	+	603	WP_234949564.1	PP2C family serine/threonine-protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T232081 WP_003156187.1 NZ_CP090905:472963-473313 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T232081 NZ_CP127252:c186627-186397 [Escherichia coli]
CTGGATCAGCTATTTAAATCACCTTTACCTCAAGGAATCAAATGGACTGATATAGAATCTCTGGTTAAAGCGTTAGGCGG
AGAAATTAAGGAAGGTAGGGGGTCGCGCTGTAAGTTCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGT
CGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTGCGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT232081 WP_003156188.1 NZ_CP090905:472676-472957 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT232081 NZ_CP127252:c186400-186041 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5