Detailed information of TA system TA232072

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pezAT/zeta-couple_hipB
• Location: 979943..981180
• Replicon: chromosome
• Accession: NZ_CP090889
• Organism: Streptococcus pneumoniae strain CH2439

Toxin (Protein)

• Gene name: pezT
• Uniprot ID: A0A4J1ZCF4
• Locus tag: L1A13_RS05070
• Protein ID: WP_000405368.1
• Coordinates: 980419..981180 (+)
• Length: 254 a.a.

Antitoxin (Protein)

• Gene name: pezA
• Uniprot ID: -
• Locus tag: L1A13_RS05065
• Protein ID: WP_000579602.1
• Coordinates: 979943..980419 (+)
• Length: 159 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L1A13_RS05045 (L1A13_05045)	975016..975870	+	855	WP_000900019.1	ADP-ribosylglycohydrolase family protein	-
L1A13_RS05050 (L1A13_05050)	975982..976866	+	885	WP_000710115.1	diacylglycerol kinase family lipid kinase	-
L1A13_RS05055 (L1A13_05055)	977029..978768	+	1740	WP_000423464.1	glycoside hydrolase family 13 protein	-
L1A13_RS05060 (L1A13_05060)	979043..979498	+	456	WP_000500478.1	hypothetical protein	-
L1A13_RS05065 (L1A13_05065)	979943..980419	+	477	WP_000579602.1	type II toxin-antitoxin system antitoxin PezA	Antitoxin
L1A13_RS05070 (L1A13_05070)	980419..981180	+	762	WP_000405368.1	type II toxin-antitoxin system toxin PezT	Toxin
L1A13_RS05075 (L1A13_05075)	981208..982365	+	1158	WP_001213951.1	hypothetical protein	-
L1A13_RS05080 (L1A13_05080)	983197..983556	+	360	WP_000529545.1	SAG1252 family conjugative relaxosome accessory protein	-
L1A13_RS05085 (L1A13_05085)	983566..983931	+	366	WP_000263039.1	plasmid mobilization relaxosome protein MobC	-
L1A13_RS05090 (L1A13_05090)	983918..985768	+	1851	Protein_997	SAG1250 family conjugative relaxase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	964687..1010160	45473

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (25-215)

Antitoxin

• Cro/C1-type helix-turn-helix domain (3-60)

Sequences

Toxin

Protein

• Length: 254 a.a.
• Molecular weight: 29185.27 Da
• Isoelectric Point: 7.5090

>T232072 WP_000405368.1 NZ_CP090889:980419-981180 [Streptococcus pneumoniae]
MEIQDYTDSEFKHALARNLRSLTRGKKSSKQPIAILLGGQSGAGKTTIHRIKQKEFQGNIVIIDGDSFRSQHPHYLELQQ
EYGKDSVEYTKDFVGKMVESLVTKLSSLRYNLLIEGTLRTVDVPKKTAQLLKNKGYEVQLALIATKPELSYLSTLIRYEE
LYIINPNQARATPKEHHDFIVNHLVDNTRQLEELAIFERIQIYQRDRSCVYDSKENTTSAADVLQELLFGEWSQVEKEML
RVGEKRLNELLEK

Nucleotide

• Length: 762 bp

>T232072 NZ_CP127237:159234-159725 [Klebsiella pneumoniae]
GTGGGCAAAGTAACGGCTCCGGTCCCACTCGGTACGTCACACATCCTCGCTGAGTTTCACTGTGGTGAGCCTGTACTGGA
CGAATGGATAAAGCACCGGGGACTAAAAAATCAGTCCCTGGGGGCGGCCAGAACGTTTGTGGTGTGCCGGGGAAATGCTT
CACAGGTGCTTGCCTACTACTCTCTCGCGACCGGCAGCGTCACTCATGCCATTGCGCCGGGAAGCCTGCGAAGGAATATG
CCCGATCCTGTACCTGTCATCATCCTGGCCCGTCTTGCTGTCGATACGAGTTATCACGGCCAGGGACTGGGGGCTGATTT
ACTGCATGATGCCGTATTACGTTGCTGTCGGGTGGCTGAGAACATTGGCGTGCGGGCCGTTATGGTTCATGCACTCTCTG
ACAGTGCCAGACAGTTCTACATACATCACGGATTTATCCCTTCACAAACGCAGGATAGAACCCTTTTTTTACGGTTGCCT
CTCATTACTTAG

Antitoxin

Protein

• Length: 159 a.a.
• Molecular weight: 18289.70 Da
• Isoelectric Point: 4.4188

>AT232072 WP_000579602.1 NZ_CP090889:979943-980419 [Streptococcus pneumoniae]
MIGKNIKSLRKTHDLTQHEFARIIGISRNSLSRYENGTSSVSTELIDIICQKFNVSYVDIVGEDKMLNPVEDYELTLKIE
IVKERGANLLSRLYRYQDSQGISIDDESNPWILMSDDLSDLIQTNIYLVENFDEIERYSGYLDGIERMLEISEKRMVA

Nucleotide

• Length: 477 bp

>AT232072 NZ_CP127237:158980-159246 [Klebsiella pneumoniae]
ATGAAATCTGATGTTCAGCTCAACATCCGGGCGAAAGAAGCCCAGCGGGCTCTGATTGATACTGCCGCTGAGATTCTCCA
CAAATCACGCACTGATTTTATACTGGAGATGGCCTGTCAGGCGGCGGAAAACGTCATTCTTGACCGCCGTATGTTCAATC
TCAATGATGAACAGTACGCCGAGTTTATCGACATGCTGGATGCGCCGGTCACCGCTAACCCGGCGATGGAGAAACTCCTG
GCGAGAAAACCGCAGTGGGCAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A4J1ZCF4