Detailed information of TA system TA232069

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pezAT/zeta-couple_hipB
• Location: 972851..974088
• Replicon: chromosome
• Accession: NZ_CP090888
• Organism: Streptococcus pneumoniae strain PT8465

Toxin (Protein)

• Gene name: pezT
• Uniprot ID: A0A4J1ZCF4
• Locus tag: L1A19_RS04895
• Protein ID: WP_000405368.1
• Coordinates: 973327..974088 (+)
• Length: 254 a.a.

Antitoxin (Protein)

• Gene name: pezA
• Uniprot ID: -
• Locus tag: L1A19_RS04890
• Protein ID: WP_000579602.1
• Coordinates: 972851..973327 (+)
• Length: 159 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L1A19_RS04870 (L1A19_04870)	967924..968778	+	855	WP_000900019.1	ADP-ribosylglycohydrolase family protein	-
L1A19_RS04875 (L1A19_04875)	968890..969774	+	885	WP_000710115.1	diacylglycerol kinase family lipid kinase	-
L1A19_RS04880 (L1A19_04880)	969937..971676	+	1740	WP_000423464.1	glycoside hydrolase family 13 protein	-
L1A19_RS04885 (L1A19_04885)	971951..972406	+	456	WP_000500478.1	hypothetical protein	-
L1A19_RS04890 (L1A19_04890)	972851..973327	+	477	WP_000579602.1	type II toxin-antitoxin system antitoxin PezA	Antitoxin
L1A19_RS04895 (L1A19_04895)	973327..974088	+	762	WP_000405368.1	type II toxin-antitoxin system toxin PezT	Toxin
L1A19_RS04900 (L1A19_04900)	974116..975273	+	1158	WP_001213951.1	hypothetical protein	-
L1A19_RS04905 (L1A19_04905)	976105..976464	+	360	WP_000529543.1	SAG1252 family conjugative relaxosome accessory protein	-
L1A19_RS04910 (L1A19_04910)	976474..976839	+	366	Protein_979	plasmid mobilization relaxosome protein MobC	-
L1A19_RS04915 (L1A19_04915)	976826..978676	+	1851	WP_061630439.1	SAG1250 family conjugative relaxase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (25-215)

Antitoxin

• Cro/C1-type helix-turn-helix domain (3-60)

Sequences

Toxin

Protein

• Length: 254 a.a.
• Molecular weight: 29185.27 Da
• Isoelectric Point: 7.5090

>T232069 WP_000405368.1 NZ_CP090888:973327-974088 [Streptococcus pneumoniae]
MEIQDYTDSEFKHALARNLRSLTRGKKSSKQPIAILLGGQSGAGKTTIHRIKQKEFQGNIVIIDGDSFRSQHPHYLELQQ
EYGKDSVEYTKDFVGKMVESLVTKLSSLRYNLLIEGTLRTVDVPKKTAQLLKNKGYEVQLALIATKPELSYLSTLIRYEE
LYIINPNQARATPKEHHDFIVNHLVDNTRQLEELAIFERIQIYQRDRSCVYDSKENTTSAADVLQELLFGEWSQVEKEML
RVGEKRLNELLEK

Nucleotide

• Length: 762 bp

>T232069 NZ_CP127236:c135546-135397 [Klebsiella pneumoniae]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTATTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Protein

• Length: 159 a.a.
• Molecular weight: 18289.70 Da
• Isoelectric Point: 4.4188

>AT232069 WP_000579602.1 NZ_CP090888:972851-973327 [Streptococcus pneumoniae]
MIGKNIKSLRKTHDLTQHEFARIIGISRNSLSRYENGTSSVSTELIDIICQKFNVSYVDIVGEDKMLNPVEDYELTLKIE
IVKERGANLLSRLYRYQDSQGISIDDESNPWILMSDDLSDLIQTNIYLVENFDEIERYSGYLDGIERMLEISEKRMVA

Nucleotide

• Length: 477 bp

>AT232069 NZ_CP127236:135591-135650 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A4J1ZCF4