Detailed information of TA system TA232065

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pezAT/zeta-couple_hipB
• Location: 1074775..1076009
• Replicon: chromosome
• Accession: NZ_CP090887
• Organism: Streptococcus pneumoniae strain NP7536

Toxin (Protein)

• Gene name: pezT
• Uniprot ID: B6ZCD1
• Locus tag: L1A18_RS05370
• Protein ID: WP_000354294.1
• Coordinates: 1075251..1076009 (+)
• Length: 253 a.a.

Antitoxin (Protein)

• Gene name: pezA
• Uniprot ID: B6ZCD2
• Locus tag: L1A18_RS05365
• Protein ID: WP_000579617.1
• Coordinates: 1074775..1075251 (+)
• Length: 159 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L1A18_RS05325 (L1A18_05325)	1071032..1071316	+	285	WP_000969646.1	DUF5962 family protein	-
L1A18_RS05330 (L1A18_05330)	1071330..1071626	+	297	WP_000998894.1	DUF5966 family protein	-
L1A18_RS05335 (L1A18_05335)	1071640..1071837	-	198	WP_001841477.1	hypothetical protein	-
L1A18_RS05340 (L1A18_05340)	1071997..1072638	+	642	WP_000933083.1	hypothetical protein	-
L1A18_RS05345 (L1A18_05345)	1072648..1073733	+	1086	WP_000401901.1	toprim domain-containing protein	-
L1A18_RS05350 (L1A18_05350)	1073784..1074017	+	234	WP_000152419.1	DUF5965 family protein	-
L1A18_RS05355 (L1A18_05355)	1074014..1074397	+	384	WP_000159222.1	DUF5945 family protein	-
L1A18_RS05360 (L1A18_05360)	1074435..1074707	+	273	WP_000495438.1	hypothetical protein	-
L1A18_RS05365 (L1A18_05365)	1074775..1075251	+	477	WP_000579617.1	type II toxin-antitoxin system antitoxin PezA	Antitoxin
L1A18_RS05370 (L1A18_05370)	1075251..1076009	+	759	WP_000354294.1	type II toxin-antitoxin system toxin PezT	Toxin
L1A18_RS05375 (L1A18_05375)	1076485..1077273	+	789	WP_000214003.1	hypothetical protein	-
L1A18_RS05380 (L1A18_05380)	1077512..1077760	+	249	WP_001814254.1	hypothetical protein	-
L1A18_RS05385 (L1A18_05385)	1077931..1078650	+	720	Protein_1070	type A chloramphenicol O-acetyltransferase	-
L1A18_RS05390 (L1A18_05390)	1079446..1080369	-	924	WP_074189357.1	protein rep	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	tet(M) / cat(pC194)	-	1045507..1091904	46397

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (25-214)

Antitoxin

• Cro/C1-type helix-turn-helix domain (3-60)

Sequences

Toxin

Protein

• Length: 253 a.a.
• Molecular weight: 28929.87 Da
• Isoelectric Point: 7.5458

>T232065 WP_000354294.1 NZ_CP090887:1075251-1076009 [Streptococcus pneumoniae]
MDIQDYTDSEFKHALARNLRSLTRGKKSSKQPIAILLGGQSGAGKTTIHRIKQKEFQGNIVIIDGDSFRSQHPHYLELQQ
EYGKDSVEYTKYFAGKMVESLVRELSHLGYNLLIEGTLRTIDVPKKTAQLLKSRGYEIQLALIATKPKLSYLSTLIRYEE
LYAINPNQARATPKEHHDFIVNHLVDNTRQLEELAIFERIQIYQRDRGCVYDAKENTTSAADVLQELLFGEWSQVEKDML
KSGEERLKDLTN

Nucleotide

• Length: 759 bp

>T232065 NZ_CP127235:c4797044-4796511 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGTGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAAGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 159 a.a.
• Molecular weight: 18286.74 Da
• Isoelectric Point: 4.4977

>AT232065 WP_000579617.1 NZ_CP090887:1074775-1075251 [Streptococcus pneumoniae]
MIGKNIKSLRKTHDLTQPEFARIVGISRNSLSRYENGTSSVSTELIDIICQKFNVSYVDIVGEDKMLNPVEDYELTLKIE
IVKERGANLLSRLYRYQDNRGISIDDESNPWILMSDDLSDLIHTNIYLVETFDEIERYSGYLDGIERMLEISEKRMVA

Nucleotide

• Length: 477 bp

>AT232065 NZ_CP127235:c4797321-4797055 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Structures

Toxin

• AlphaFold DB: B6ZCD1

Antitoxin

• AlphaFold DB: B6ZCD2