Detailed information of TA system TA232064

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pezAT/zeta-couple_hipB
• Location: 914255..915492
• Replicon: chromosome
• Accession: NZ_CP090887
• Organism: Streptococcus pneumoniae strain NP7536

Toxin (Protein)

• Gene name: pezT
• Uniprot ID: A0A4J1ZCF4
• Locus tag: L1A18_RS04605
• Protein ID: WP_000405368.1
• Coordinates: 914731..915492 (+)
• Length: 254 a.a.

Antitoxin (Protein)

• Gene name: pezA
• Uniprot ID: -
• Locus tag: L1A18_RS04600
• Protein ID: WP_000579602.1
• Coordinates: 914255..914731 (+)
• Length: 159 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L1A18_RS04580 (L1A18_04580)	909328..910182	+	855	WP_000900019.1	ADP-ribosylglycohydrolase family protein	-
L1A18_RS04585 (L1A18_04585)	910294..911178	+	885	WP_000710115.1	diacylglycerol kinase family lipid kinase	-
L1A18_RS04590 (L1A18_04590)	911341..913080	+	1740	WP_000423464.1	glycoside hydrolase family 13 protein	-
L1A18_RS04595 (L1A18_04595)	913355..913810	+	456	WP_000500478.1	hypothetical protein	-
L1A18_RS04600 (L1A18_04600)	914255..914731	+	477	WP_000579602.1	type II toxin-antitoxin system antitoxin PezA	Antitoxin
L1A18_RS04605 (L1A18_04605)	914731..915492	+	762	WP_000405368.1	type II toxin-antitoxin system toxin PezT	Toxin
L1A18_RS04610 (L1A18_04610)	915520..916677	+	1158	WP_001213951.1	hypothetical protein	-
L1A18_RS04615 (L1A18_04615)	917509..917868	+	360	WP_000529545.1	SAG1252 family conjugative relaxosome accessory protein	-
L1A18_RS04620 (L1A18_04620)	917878..918243	+	366	WP_000263039.1	plasmid mobilization relaxosome protein MobC	-
L1A18_RS04625 (L1A18_04625)	918230..920080	+	1851	Protein_918	SAG1250 family conjugative relaxase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	885037..1038871	153834

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (25-215)

Antitoxin

• Cro/C1-type helix-turn-helix domain (3-60)

Sequences

Toxin

Protein

• Length: 254 a.a.
• Molecular weight: 29185.27 Da
• Isoelectric Point: 7.5090

>T232064 WP_000405368.1 NZ_CP090887:914731-915492 [Streptococcus pneumoniae]
MEIQDYTDSEFKHALARNLRSLTRGKKSSKQPIAILLGGQSGAGKTTIHRIKQKEFQGNIVIIDGDSFRSQHPHYLELQQ
EYGKDSVEYTKDFVGKMVESLVTKLSSLRYNLLIEGTLRTVDVPKKTAQLLKNKGYEVQLALIATKPELSYLSTLIRYEE
LYIINPNQARATPKEHHDFIVNHLVDNTRQLEELAIFERIQIYQRDRSCVYDSKENTTSAADVLQELLFGEWSQVEKEML
RVGEKRLNELLEK

Nucleotide

• Length: 762 bp

>T232064 NZ_CP127235:4184212-4184430 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 159 a.a.
• Molecular weight: 18289.70 Da
• Isoelectric Point: 4.4188

>AT232064 WP_000579602.1 NZ_CP090887:914255-914731 [Streptococcus pneumoniae]
MIGKNIKSLRKTHDLTQHEFARIIGISRNSLSRYENGTSSVSTELIDIICQKFNVSYVDIVGEDKMLNPVEDYELTLKIE
IVKERGANLLSRLYRYQDSQGISIDDESNPWILMSDDLSDLIQTNIYLVENFDEIERYSGYLDGIERMLEISEKRMVA

Nucleotide

• Length: 477 bp

>AT232064 NZ_CP127235:4183811-4184185 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: A0A4J1ZCF4