Detailed information of TA system TA232062

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pezAT/zeta-couple_hipB
• Location: 1541773..1543007
• Replicon: chromosome
• Accession: NZ_CP090886
• Organism: Streptococcus pneumoniae strain NP7513

Toxin (Protein)

• Gene name: pezT
• Uniprot ID: B6ZCD1
• Locus tag: L1A17_RS08010
• Protein ID: WP_000354294.1
• Coordinates: 1541773..1542531 (-)
• Length: 253 a.a.

Antitoxin (Protein)

• Gene name: pezA
• Uniprot ID: B6ZCD2
• Locus tag: L1A17_RS08015
• Protein ID: WP_000579617.1
• Coordinates: 1542531..1543007 (-)
• Length: 159 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L1A17_RS07990 (L1A17_07990)	1537491..1538336	+	846	WP_000366623.1	protein rep	-
L1A17_RS07995 (L1A17_07995)	1539132..1539851	-	720	Protein_1534	type A chloramphenicol O-acetyltransferase	-
L1A17_RS08000 (L1A17_08000)	1540022..1540270	-	249	WP_001814254.1	hypothetical protein	-
L1A17_RS08005 (L1A17_08005)	1540509..1541297	-	789	WP_000214003.1	hypothetical protein	-
L1A17_RS08010 (L1A17_08010)	1541773..1542531	-	759	WP_000354294.1	type II toxin-antitoxin system toxin PezT	Toxin
L1A17_RS08015 (L1A17_08015)	1542531..1543007	-	477	WP_000579617.1	type II toxin-antitoxin system antitoxin PezA	Antitoxin
L1A17_RS08020 (L1A17_08020)	1543075..1543347	-	273	WP_000495438.1	hypothetical protein	-
L1A17_RS08025 (L1A17_08025)	1543385..1543768	-	384	WP_000159213.1	DUF5945 family protein	-
L1A17_RS08030 (L1A17_08030)	1543765..1543998	-	234	WP_061746195.1	DUF5965 family protein	-
L1A17_RS08035 (L1A17_08035)	1544049..1545134	-	1086	WP_000401904.1	toprim domain-containing protein	-
L1A17_RS08040 (L1A17_08040)	1545144..1545785	-	642	WP_000933078.1	hypothetical protein	-
L1A17_RS08045 (L1A17_08045)	1545920..1546117	+	198	WP_074187117.1	hypothetical protein	-
L1A17_RS08050 (L1A17_08050)	1546131..1546427	-	297	WP_000998888.1	DUF5966 family protein	-
L1A17_RS08055 (L1A17_08055)	1546441..1546725	-	285	WP_000969645.1	DUF5962 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	cat(pC194)	-	1521725..1544604	22879

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (25-214)

Antitoxin

• Cro/C1-type helix-turn-helix domain (3-60)

Sequences

Toxin

Protein

• Length: 253 a.a.
• Molecular weight: 28929.87 Da
• Isoelectric Point: 7.5458

>T232062 WP_000354294.1 NZ_CP090886:c1542531-1541773 [Streptococcus pneumoniae]
MDIQDYTDSEFKHALARNLRSLTRGKKSSKQPIAILLGGQSGAGKTTIHRIKQKEFQGNIVIIDGDSFRSQHPHYLELQQ
EYGKDSVEYTKYFAGKMVESLVRELSHLGYNLLIEGTLRTIDVPKKTAQLLKSRGYEIQLALIATKPKLSYLSTLIRYEE
LYAINPNQARATPKEHHDFIVNHLVDNTRQLEELAIFERIQIYQRDRGCVYDAKENTTSAADVLQELLFGEWSQVEKDML
KSGEERLKDLTN

Nucleotide

• Length: 759 bp

>T232062 NZ_CP127235:2014082-2014184 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 159 a.a.
• Molecular weight: 18286.74 Da
• Isoelectric Point: 4.4977

>AT232062 WP_000579617.1 NZ_CP090886:c1543007-1542531 [Streptococcus pneumoniae]
MIGKNIKSLRKTHDLTQPEFARIVGISRNSLSRYENGTSSVSTELIDIICQKFNVSYVDIVGEDKMLNPVEDYELTLKIE
IVKERGANLLSRLYRYQDNRGISIDDESNPWILMSDDLSDLIHTNIYLVETFDEIERYSGYLDGIERMLEISEKRMVA

Nucleotide

• Length: 477 bp

>AT232062 NZ_CP127235:c2014191-2014046 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Structures

Toxin

• AlphaFold DB: B6ZCD1

Antitoxin

• AlphaFold DB: B6ZCD2