Detailed information of TA system TA232054

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pezAT/zeta-couple_hipB
• Location: 914637..915874
• Replicon: chromosome
• Accession: NZ_CP090885
• Organism: Streptococcus pneumoniae strain ND6401

Toxin (Protein)

• Gene name: pezT
• Uniprot ID: A0A4J1ZCF4
• Locus tag: L1A16_RS04620
• Protein ID: WP_000405368.1
• Coordinates: 915113..915874 (+)
• Length: 254 a.a.

Antitoxin (Protein)

• Gene name: pezA
• Uniprot ID: -
• Locus tag: L1A16_RS04615
• Protein ID: WP_000579602.1
• Coordinates: 914637..915113 (+)
• Length: 159 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L1A16_RS04595 (L1A16_04595)	909710..910564	+	855	WP_000900019.1	ADP-ribosylglycohydrolase family protein	-
L1A16_RS04600 (L1A16_04600)	910676..911560	+	885	WP_000710115.1	diacylglycerol kinase family lipid kinase	-
L1A16_RS04605 (L1A16_04605)	911723..913462	+	1740	WP_000423464.1	glycoside hydrolase family 13 protein	-
L1A16_RS04610 (L1A16_04610)	913737..914192	+	456	WP_000500478.1	hypothetical protein	-
L1A16_RS04615 (L1A16_04615)	914637..915113	+	477	WP_000579602.1	type II toxin-antitoxin system antitoxin PezA	Antitoxin
L1A16_RS04620 (L1A16_04620)	915113..915874	+	762	WP_000405368.1	type II toxin-antitoxin system toxin PezT	Toxin
L1A16_RS04625 (L1A16_04625)	915902..917059	+	1158	WP_001213951.1	hypothetical protein	-
L1A16_RS04630 (L1A16_04630)	917891..918250	+	360	WP_000529545.1	SAG1252 family conjugative relaxosome accessory protein	-
L1A16_RS04635 (L1A16_04635)	918260..918625	+	366	WP_000263039.1	plasmid mobilization relaxosome protein MobC	-
L1A16_RS04640 (L1A16_04640)	918612..920462	+	1851	Protein_924	SAG1250 family conjugative relaxase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (25-215)

Antitoxin

• Cro/C1-type helix-turn-helix domain (3-60)

Sequences

Toxin

Protein

• Length: 254 a.a.
• Molecular weight: 29185.27 Da
• Isoelectric Point: 7.5090

>T232054 WP_000405368.1 NZ_CP090885:915113-915874 [Streptococcus pneumoniae]
MEIQDYTDSEFKHALARNLRSLTRGKKSSKQPIAILLGGQSGAGKTTIHRIKQKEFQGNIVIIDGDSFRSQHPHYLELQQ
EYGKDSVEYTKDFVGKMVESLVTKLSSLRYNLLIEGTLRTVDVPKKTAQLLKNKGYEVQLALIATKPELSYLSTLIRYEE
LYIINPNQARATPKEHHDFIVNHLVDNTRQLEELAIFERIQIYQRDRSCVYDSKENTTSAADVLQELLFGEWSQVEKEML
RVGEKRLNELLEK

Nucleotide

• Length: 762 bp

>T232054 NZ_CP127231:57288-57704 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 159 a.a.
• Molecular weight: 18289.70 Da
• Isoelectric Point: 4.4188

>AT232054 WP_000579602.1 NZ_CP090885:914637-915113 [Streptococcus pneumoniae]
MIGKNIKSLRKTHDLTQHEFARIIGISRNSLSRYENGTSSVSTELIDIICQKFNVSYVDIVGEDKMLNPVEDYELTLKIE
IVKERGANLLSRLYRYQDSQGISIDDESNPWILMSDDLSDLIQTNIYLVENFDEIERYSGYLDGIERMLEISEKRMVA

Nucleotide

• Length: 477 bp

>AT232054 NZ_CP127231:57061-57291 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A4J1ZCF4