Detailed information of TA system TA232048

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pezAT/zeta-couple_hipB
• Location: 980368..981605
• Replicon: chromosome
• Accession: NZ_CP090883
• Organism: Streptococcus pneumoniae strain LE4448

Toxin (Protein)

• Gene name: pezT
• Uniprot ID: A0A4J1ZCF4
• Locus tag: L1A14_RS05075
• Protein ID: WP_000405368.1
• Coordinates: 980844..981605 (+)
• Length: 254 a.a.

Antitoxin (Protein)

• Gene name: pezA
• Uniprot ID: -
• Locus tag: L1A14_RS05070
• Protein ID: WP_000579602.1
• Coordinates: 980368..980844 (+)
• Length: 159 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L1A14_RS05050 (L1A14_05050)	975441..976295	+	855	WP_000900019.1	ADP-ribosylglycohydrolase family protein	-
L1A14_RS05055 (L1A14_05055)	976407..977291	+	885	WP_000710115.1	diacylglycerol kinase family lipid kinase	-
L1A14_RS05060 (L1A14_05060)	977454..979193	+	1740	WP_000423464.1	glycoside hydrolase family 13 protein	-
L1A14_RS05065 (L1A14_05065)	979468..979923	+	456	WP_000500478.1	hypothetical protein	-
L1A14_RS05070 (L1A14_05070)	980368..980844	+	477	WP_000579602.1	type II toxin-antitoxin system antitoxin PezA	Antitoxin
L1A14_RS05075 (L1A14_05075)	980844..981605	+	762	WP_000405368.1	type II toxin-antitoxin system toxin PezT	Toxin
L1A14_RS05080 (L1A14_05080)	981633..982790	+	1158	WP_001213951.1	hypothetical protein	-
L1A14_RS05085 (L1A14_05085)	983622..983981	+	360	WP_000529545.1	SAG1252 family conjugative relaxosome accessory protein	-
L1A14_RS05090 (L1A14_05090)	983991..984356	+	366	WP_000263039.1	plasmid mobilization relaxosome protein MobC	-
L1A14_RS05095 (L1A14_05095)	984343..986193	+	1851	Protein_998	SAG1250 family conjugative relaxase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	965112..1010578	45466

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (25-215)

Antitoxin

• Cro/C1-type helix-turn-helix domain (3-60)

Sequences

Toxin

Protein

• Length: 254 a.a.
• Molecular weight: 29185.27 Da
• Isoelectric Point: 7.5090

>T232048 WP_000405368.1 NZ_CP090883:980844-981605 [Streptococcus pneumoniae]
MEIQDYTDSEFKHALARNLRSLTRGKKSSKQPIAILLGGQSGAGKTTIHRIKQKEFQGNIVIIDGDSFRSQHPHYLELQQ
EYGKDSVEYTKDFVGKMVESLVTKLSSLRYNLLIEGTLRTVDVPKKTAQLLKNKGYEVQLALIATKPELSYLSTLIRYEE
LYIINPNQARATPKEHHDFIVNHLVDNTRQLEELAIFERIQIYQRDRSCVYDSKENTTSAADVLQELLFGEWSQVEKEML
RVGEKRLNELLEK

Nucleotide

• Length: 762 bp

>T232048 NZ_CP127229:2658422-2658769 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTGTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGTGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 159 a.a.
• Molecular weight: 18289.70 Da
• Isoelectric Point: 4.4188

>AT232048 WP_000579602.1 NZ_CP090883:980368-980844 [Streptococcus pneumoniae]
MIGKNIKSLRKTHDLTQHEFARIIGISRNSLSRYENGTSSVSTELIDIICQKFNVSYVDIVGEDKMLNPVEDYELTLKIE
IVKERGANLLSRLYRYQDSQGISIDDESNPWILMSDDLSDLIQTNIYLVENFDEIERYSGYLDGIERMLEISEKRMVA

Nucleotide

• Length: 477 bp

>AT232048 NZ_CP127229:2658769-2659068 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Structures

Toxin

• AlphaFold DB: A0A4J1ZCF4