Detailed information of TA system TA232045

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): zeta-pezA/zeta-couple_hipB
• Location: 1080783..1082017
• Replicon: chromosome
• Accession: NZ_CP090882
• Organism: Streptococcus pneumoniae strain CH2241

Toxin (Protein)

• Gene name: zeta
• Uniprot ID: B6ZCC3
• Locus tag: L1A12_RS05400
• Protein ID: WP_000405371.1
• Coordinates: 1081259..1082017 (+)
• Length: 253 a.a.

Antitoxin (Protein)

• Gene name: pezA
• Uniprot ID: B6ZCC4
• Locus tag: L1A12_RS05395
• Protein ID: WP_000579612.1
• Coordinates: 1080783..1081259 (+)
• Length: 159 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L1A12_RS05370 (L1A12_05370)	1075893..1076141	+	249	WP_001814254.1	hypothetical protein	-
L1A12_RS05375 (L1A12_05375)	1076312..1077031	+	720	Protein_1068	type A chloramphenicol O-acetyltransferase	-
L1A12_RS05380 (L1A12_05380)	1077827..1078750	-	924	WP_074189357.1	protein rep	-
L1A12_RS05385 (L1A12_05385)	1078954..1079784	+	831	Protein_1070	alpha-amylase family glycosyl hydrolase	-
L1A12_RS05390 (L1A12_05390)	1080052..1080507	+	456	WP_025173603.1	hypothetical protein	-
L1A12_RS05395 (L1A12_05395)	1080783..1081259	+	477	WP_000579612.1	type II toxin-antitoxin system antitoxin PezA	Antitoxin
L1A12_RS05400 (L1A12_05400)	1081259..1082017	+	759	WP_000405371.1	type II toxin-antitoxin system toxin PezT	Toxin
L1A12_RS05405 (L1A12_05405)	1082281..1082964	+	684	WP_000492702.1	LexA family transcriptional regulator	-
L1A12_RS05410 (L1A12_05410)	1082967..1084382	+	1416	WP_000219662.1	Y-family DNA polymerase	-
L1A12_RS05415 (L1A12_05415)	1084382..1084744	+	363	WP_001082151.1	hypothetical protein	-
L1A12_RS05420 (L1A12_05420)	1084734..1085024	+	291	WP_001080183.1	DUF5960 family protein	-
L1A12_RS05425 (L1A12_05425)	1085319..1085675	+	357	WP_000827835.1	SAG1252 family conjugative relaxosome accessory protein	-
L1A12_RS05430 (L1A12_05430)	1085680..1086045	+	366	WP_000401236.1	MobC family plasmid mobilization relaxosome protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1079863..1090285	10422
	-	inside	Integrative and Conjugative Element	erm(B) / tet(M) / cat(pC194)	-	1018481..1079612	61131

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (25-215)

Antitoxin

• Cro/C1-type helix-turn-helix domain (3-60)

Sequences

Toxin

Protein

• Length: 253 a.a.
• Molecular weight: 28812.66 Da
• Isoelectric Point: 8.0883

>T232045 WP_000405371.1 NZ_CP090882:1081259-1082017 [Streptococcus pneumoniae]
VEIQDYTDSEFKHALARNLRSLTRGKKSSKQPIAILLGGQSGAGKTTIHRIKQKEFQGNIVIIDGDSFRSQHPHYSGLQQ
EYGKDSVEYTKDFAGKMVESLVTELSHLGYNLLIEGTLRTIDVPKKTAQLLKSRGYEVQLALIATKPKLSYLSTLIRYEE
LYAINPNQARATPKEHHDFIVNHLVDNTRQLEELAIFERIQIYQRDRSCVYDSKENTTSAATVLHYLLFGEWNQVEKEML
KSGEERLKDLTN

Nucleotide

• Length: 759 bp

>T232045 NZ_CP127229:c1904539-1903622 [Klebsiella pneumoniae]
ATGGATATACAGGCGCCTTATTATCGGCAGGTTGCTCTGCTAATGCAGGTTTTACCCTATGTTGCTGTCGAACGGGAATT
TGCGCTGAAAGGTGGCACTGCCATCAATCTCTTTATCCGCGATTTCCCTCGATTATCGGTGGATATCGACCTCGCCTGGG
TGCCCCTGGAAAGCAGAGCCATCGCTTTACCTCATATACGGGATGCGCTTGCACGTATTGCCGCCAATCTGCAACAACAG
GCAGGTATGAGCGCTGTATTACAAGCTAATCGTTCAGATGAGATGCGTGTCATTGTTACTACCGATAGCGCGCAAATAAA
GATAGAGGTTTCACCGGTCGCCCGGGGAACATTATATCCACCACAGGAGCGAGAAGTGGTGGGGGCGGTAGAGGACGTTT
TTGGCTATGCCAGCCTTCCAGTGGTTTCGTTGCCTGACCTCTACGGTGGAAAATTATGCGCGGCGCTGGATCGCCAGCAT
CCCAGAGATTTTTATGACGTGAAATTACTTCTTGATGCGCAGGAGCTTGACCGTCCCATTTTCAATGGGTTTATCGTGTA
TTTACTGAGCCATAATCGGCCTCTGGCTGAAGTGCTGAATCCTCGCTGGAAAGATATTGCTGAACCTTTTTACCGTGAAT
TCAGCGGGATGACATTTGAGACGATAGCCCTGGAGGAACTGACAGCAGTCCCCAACAGGATGATTGCTGCGTTAAAATCC
TGTTTCACACAACAGGATGTCGATTTTTTACTGTCATTTAAACGAGGCGAACCAGACTGGCGTTTAGCGCCGGAAATGCG
TATTCAGGATTTGCCTGCCGTGCAGTGGAAATTGCGCAATATTCATCAGATGCCAGCGATAAAACGCGCTGAGTCATTAG
ATAAACTGGAAAAGGTGCTGGCCGAGTGGCGCAGTTAG

Antitoxin

Protein

• Length: 159 a.a.
• Molecular weight: 18245.68 Da
• Isoelectric Point: 4.4188

>AT232045 WP_000579612.1 NZ_CP090882:1080783-1081259 [Streptococcus pneumoniae]
MIGKNIKSLRKTHDLTQPEFARIIGISRNSLSRYENGTSSVSTELIDIICQKFNVSYVDIVGEDKMLNPVEDYELTLKIE
IVKERGANLLSRLYRYQDSQGISIDDESNPWILMSDDLSDLIHTNIYLVETFDEIERYSGYLDGIERMLEISEKRMVA

Nucleotide

• Length: 477 bp

>AT232045 NZ_CP127229:c1905303-1904545 [Klebsiella pneumoniae]
ATGGCAACAAAATTAAACTGGCTGCTGCAAAACAGCGCTCCCGGAGATGTGATCCTTCAGTCCTGGTTAAGCCAGCATGC
CATCTCTCCCTCTCTGTCCTTTAAATACGCGCAAAGTGGCTGGTTAAAAAAAAGAGGCAACGGCGTATATGCGCGTGCGG
GACGTGAGCCGGAGTGGAACGATGCATTAGCATGTTTGCAAAATCAGCTTGCCGCTCCGGTGTATGTTGCGGGGCTCAGT
AGCTTAGTCTGGCAGGGCCGTTCACACTATCTACAGCTAAAACAAAACCAGTGCTGGCTTAGTGTGGAAAATAAAGCGCT
GCTGCCGAAATGGTTCAGGGAATTCCCGGGTGTGGAGTGGATAGTCATATCGGGGAAAAAACTGCCGGTGCTGGATGATA
GATACCGTGTAACGCTTGATATAAAAGGGAAAAAACTCATCGGCAGTGCGCCAGAACTGGCTGCTTACGAGTTGCTGAGC
GCGGTGCCGGGGACCCTCTCATTTAACCACGCTGCAGAGCTTTTCCAGGGGCTGGTTAATCTTAATCCTCGTAAAGTAGA
GTATCTTCTTTCCGTCAGCCAGTCCGTGCAGGCCAAGCGTTTGTATCTCTTCTTCGCCAGTTTTTACGAGCATGGCTGGC
TCAAACGAATCGATAGCCAAAAAATCGATCTGGGAGCGGGCAAGCGGCAAATCGTGGAAAATGGAAAGTTCAATGCTCAA
TACCAAATCACAGTGCCTGAACGCTTTCAAAAGGAATAA

Structures

Toxin

• AlphaFold DB: B6ZCC3

Antitoxin

• AlphaFold DB: B6ZCC4