Detailed information of TA system TA232015

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tsbAT/-
• Location: 2214738..2215514
• Replicon: chromosome
• Accession: NZ_CP090877
• Organism: Staphylococcus aureus strain SAUR_BFS11

Toxin (Protein)

• Gene name: tsbT
• Uniprot ID: X5E2E6
• Locus tag: L1A06_RS10995
• Protein ID: WP_000031108.1
• Coordinates: 2215362..2215514 (+)
• Length: 51 a.a.

Antitoxin (Protein)

• Gene name: tsbA
• Uniprot ID: W8U4V4
• Locus tag: L1A06_RS10990
• Protein ID: WP_001251224.1
• Coordinates: 2214738..2215337 (+)
• Length: 200 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L1A06_RS10970 (2210655)	2210655..2212112	+	1458	WP_000649908.1	ABC transporter permease subunit	-
L1A06_RS10975 (2212105)	2212105..2212827	+	723	WP_000590809.1	amino acid ABC transporter ATP-binding protein	-
L1A06_RS10980 (2212978)	2212978..2214105	+	1128	WP_000379985.1	tRNA epoxyqueuosine(34) reductase QueG	-
L1A06_RS10985 (2214110)	2214110..2214580	+	471	WP_000181394.1	tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL	-
L1A06_RS10990 (2214738)	2214738..2215337	+	600	WP_001251224.1	glucosamine-6-phosphate isomerase	Antitoxin
L1A06_RS10995 (2215362)	2215362..2215514	+	153	WP_000031108.1	SAS053 family protein	Toxin
L1A06_RS11000 (2216408)	2216408..2216803	+	396	WP_000901021.1	hypothetical protein	-
L1A06_RS11005 (2216999)	2216999..2218384	+	1386	WP_000116224.1	class II fumarate hydratase	-
L1A06_RS11010 (2218845)	2218845..2219666	-	822	WP_000669376.1	RluA family pseudouridine synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Glucosamine-6-phosphate isomerase (124-196)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5936.28 Da
• Isoelectric Point: 3.8962

>T232015 WP_000031108.1 NZ_CP090877:2215362-2215514 [Staphylococcus aureus]
MSKDKDPKLNYHEEENSMVTDFEDLKELGKEMEQISDQNDQEKNSEEDSQ

Nucleotide

• Length: 153 bp

>T232015 NZ_CP127160:792930-793061 [Enterococcus sp. FZMF]
ATGGCGAATATTACTGACTTCACCGAAAAGCAATTTGAAGATCGTTTAGAAAAGAATGTTGAACGACTAACTAAAAATAG
ACTAGCGGTTGAATCGCCAACCGCTTTTTTAGGTTCTGTTGCAAAGTTTTAA

Antitoxin

Protein

• Length: 200 a.a.
• Molecular weight: 22343.47 Da
• Isoelectric Point: 5.1445

>AT232015 WP_001251224.1 NZ_CP090877:2214738-2215337 [Staphylococcus aureus]
MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDQAPVLDELKKNVEKHAVDFSQINILDYDDKKSYFEALGV
PAGQVYPIAYEKDAIELIADKIKTKENKGKLTLQVVSIDEQGKLNVSIRQGLMEAREIFLVVTGANKRDVVEKLYQENGK
TSFEPADLKAHRMVNVILDKEAAAGLPEDVKAYFTSRFA

Nucleotide

• Length: 600 bp

>AT232015 NZ_CP127160:792656-792928 [Enterococcus sp. FZMF]
ATGGCAGTTACGTATGAAAAAACATTTGAAATAGAGATCATTAACGAATTATCGGCAAGCGTTTATAATCGAGTATTAAA
CTATGTTTTGAACCATGAACTAGATACTAAAAATACTCGTTTACTAGAAGTGAATCTTTTAAATCAATTAGAAGTGGCAC
AAGAAGTTGATTTATTTCAACAACCATTTGAAGAATTACAAGCTATTCATGAGTATTGGCGGTCAATGAATCAATATTCA
AAACAAATTTTGACAAAAGAGAAAGTGGCTTAA

Structures

Toxin

• AlphaFold DB: X5E2E6

Antitoxin

• AlphaFold DB: W8U4V4