Detailed information of TA system TA232014

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2057124..2057653
• Replicon: chromosome
• Accession: NZ_CP090877
• Organism: Staphylococcus aureus strain SAUR_BFS11

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: L1A06_RS10060
• Protein ID: WP_000621175.1
• Coordinates: 2057291..2057653 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: L1A06_RS10055
• Protein ID: WP_000948331.1
• Coordinates: 2057124..2057294 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L1A06_RS10025 (2052160)	2052160..2052720	+	561	WP_001092409.1	K(+)-transporting ATPase subunit C	-
L1A06_RS10030 (2052929)	2052929..2053408	+	480	WP_001287079.1	hypothetical protein	-
L1A06_RS10035 (2053401)	2053401..2054984	+	1584	WP_001294620.1	PH domain-containing protein	-
L1A06_RS10040 (2054971)	2054971..2055462	+	492	WP_001205912.1	PH domain-containing protein	-
L1A06_RS10045 (2055466)	2055466..2055825	+	360	WP_000581202.1	holo-ACP synthase	-
L1A06_RS10050 (2055891)	2055891..2057039	+	1149	WP_000130547.1	alanine racemase	-
L1A06_RS10055 (2057124)	2057124..2057294	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
L1A06_RS10060 (2057291)	2057291..2057653	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
L1A06_RS10065 (2058002)	2058002..2059003	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
L1A06_RS10070 (2059122)	2059122..2059448	+	327	WP_001052491.1	anti-sigma factor antagonist	-
L1A06_RS10075 (2059450)	2059450..2059929	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
L1A06_RS10080 (2059904)	2059904..2060674	+	771	WP_001041107.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T232014 WP_000621175.1 NZ_CP090877:2057291-2057653 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T232014 NZ_CP127160:474412-474678 [Enterococcus sp. FZMF]
ATGAGGAGTGATACGGTCACAATCAAAAACTCTGCGAAAGTGGATCTAAGGAAAATCAAACAATCGAATTTAAAAAAACA
GTTTGAAGAAGTGATTCAGGCCTTGAAGGAAGATCCATATATGCCCACGCAGTCTTTTGAAAAGCTGAGACCTACACATG
AAGGCAGATATTCACGTAGGCTGAGTAGGCAACATCGAGTGGTTTACAAAGTTGATGAGAAAAATAAAGTTGTGGAAATT
TATTCTGCATGGACACATTATGAATGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT232014 WP_000948331.1 NZ_CP090877:2057124-2057294 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT232014 NZ_CP127160:474149-474412 [Enterococcus sp. FZMF]
ATGGCTAAAACAACGATGAATCCTAGCACTGCTCGTAAAAATTTTTATCAGTTATTGAAAGATGTAAATGAAAACCATAC
TGAAATTGAGATTATTAGTGATCGAAGTGGAAACAATGCGGTGTTGATTGGGCTAGGAGACTGGAGAGCGATTCAAGAAA
CCCTTTTTCTTGAGCAAACAGGTACTCTAGAAAAGGTACGAGACCGCGAAAAAGACGACAGTGGCTTTACGGATATCGAT
GATATTGATTGGCAAGCTCTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1