Detailed information of TA system TA231990

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 159714..160243
• Replicon: chromosome
• Accession: NZ_CP090876
• Organism: Staphylococcus aureus strain SAUR_BFS12

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: L0998_RS00750
• Protein ID: WP_000621175.1
• Coordinates: 159881..160243 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: L0998_RS00745
• Protein ID: WP_000948331.1
• Coordinates: 159714..159884 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0998_RS00715 (154750)	154750..155310	+	561	WP_054190433.1	K(+)-transporting ATPase subunit C	-
L0998_RS00720 (155519)	155519..155998	+	480	WP_054190432.1	hypothetical protein	-
L0998_RS00725 (155991)	155991..157574	+	1584	WP_054190431.1	PH domain-containing protein	-
L0998_RS00730 (157561)	157561..158052	+	492	WP_054190430.1	PH domain-containing protein	-
L0998_RS00735 (158056)	158056..158415	+	360	WP_000581197.1	holo-ACP synthase	-
L0998_RS00740 (158481)	158481..159629	+	1149	WP_054190429.1	alanine racemase	-
L0998_RS00745 (159714)	159714..159884	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
L0998_RS00750 (159881)	159881..160243	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
L0998_RS00755 (160593)	160593..161594	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
L0998_RS00760 (161713)	161713..162039	+	327	WP_001052491.1	anti-sigma factor antagonist	-
L0998_RS00765 (162041)	162041..162520	+	480	WP_054190427.1	anti-sigma B factor RsbW	-
L0998_RS00770 (162495)	162495..163265	+	771	WP_001041107.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T231990 WP_000621175.1 NZ_CP090876:159881-160243 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T231990 NZ_CP127126:c141281-141000 [Pseudomonas aeruginosa PA14]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCCCTGAAAGCCGTCCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCTTTTCGGGTAAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT231990 WP_000948331.1 NZ_CP090876:159714-159884 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT231990 NZ_CP127126:c141505-141278 [Pseudomonas aeruginosa PA14]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCGTATCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAGCACGCCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1