Detailed information of TA system TA023198

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 327267..327994
• Replicon: chromosome
• Accession: NC_011415
• Organism: Escherichia coli SE11

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: A0A979H205
• Locus tag: ECSE_RS01555
• Protein ID: WP_000854674.1
• Coordinates: 327629..327994 (+)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: A0A979H0L0
• Locus tag: ECSE_RS01550
• Protein ID: WP_000842923.1
• Coordinates: 327267..327608 (+)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECSE_RS26300	322797..323501	+	705	WP_000083853.1	WYL domain-containing protein	-
ECSE_RS01515	323501..324070	+	570	WP_000722972.1	hypothetical protein	-
ECSE_RS01520	324107..324559	+	453	WP_000737933.1	hypothetical protein	-
ECSE_RS01525	324556..325005	+	450	WP_000734140.1	hypothetical protein	-
ECSE_RS01530	325077..325307	+	231	WP_001114680.1	DUF905 domain-containing protein	-
ECSE_RS01535	325406..326227	+	822	WP_001234416.1	DUF945 domain-containing protein	-
ECSE_RS01540	326258..326701	+	444	WP_001093282.1	antirestriction protein	-
ECSE_RS01545	326714..327256	+	543	WP_001090453.1	DNA repair protein RadC	-
ECSE_RS01550	327267..327608	+	342	WP_000842923.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
ECSE_RS01555	327629..327994	+	366	WP_000854674.1	TA system toxin CbtA family protein	Toxin
ECSE_RS01560	328321..328659	-	339	Protein_296	LysR family transcriptional regulator	-
ECSE_RS01565	328710..329666	-	957	WP_000121326.1	molybdenum cofactor insertion chaperone PaoD	-
ECSE_RS01570	329676..331874	-	2199	WP_000667026.1	aldehyde oxidoreductase molybdenum-binding subunit PaoC	-
ECSE_RS01575	331871..332827	-	957	WP_000643328.1	aldehyde oxidoreductase FAD-binding subunit PaoB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	yagV/ecpE / yagW/ecpD / yagX/ecpC / yagY/ecpB / yagZ/ecpA / ykgK/ecpR / fdeC	315173..365627	50454

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (13-112)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13593.61 Da
• Isoelectric Point: 8.0503

>T23198 WP_000854674.1 NC_011415:327629-327994 [Escherichia coli SE11]
MKTLPATISRAAEPGLSPVGVWQTLLTRLMEQHYGLTLNDTPFSDETVIKEHIDAGITLANAINFLVEKYELVRIDRNGF
TSQVQAPYLTATDILHARRACGLMTRFSYREVSNIVLSRSR

Nucleotide

• Length: 366 bp

>T23198 NC_011415:327629-327994 [Escherichia coli SE11]
ATGAAAACTCTACCTGCTACAATTTCGCGCGCGGCGGAACCCGGCCTGTCGCCAGTGGGTGTCTGGCAAACTTTACTGAC
ACGCCTGATGGAACAGCACTATGGTCTGACACTGAACGATACGCCGTTCAGTGATGAAACCGTTATAAAGGAACATATCG
ATGCCGGTATCACTCTCGCTAATGCCATTAACTTTCTGGTAGAGAAATATGAGTTAGTTCGTATTGATCGCAATGGATTT
ACCTCTCAGGTACAAGCACCATATTTGACTGCGACAGACATTCTTCATGCCAGAAGAGCTTGCGGACTGATGACTCGTTT
TTCTTACCGGGAAGTAAGTAATATAGTTCTTAGCCGATCGCGGTAG

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12745.47 Da
• Isoelectric Point: 6.9370

>AT23198 WP_000842923.1 NC_011415:327267-327608 [Escherichia coli SE11]
MKSTDSEYDSFLKWGLNRNVTPCFGARLVQEGNRLHYLADRASITGKFSEAECLKLNVAFPHFISQMESMLTTGEMNPRH
VHCVTLYHNGFACEADTLGSCGYVYIAVYPSQR

Nucleotide

• Length: 342 bp

>AT23198 NC_011415:327267-327608 [Escherichia coli SE11]
ATGAAATCTACTGACTCAGAGTACGATTCATTTCTCAAGTGGGGTCTGAATCGTAATGTGACACCTTGCTTTGGCGCTCG
TCTGGTACAGGAGGGAAACCGGCTGCACTATCTGGCTGACCGGGCCAGTATCACCGGTAAGTTCAGCGAAGCAGAATGTC
TTAAGCTGAATGTGGCATTCCCACATTTTATCAGCCAGATGGAGTCGATGTTGACTACTGGTGAAATGAATCCCCGCCAT
GTCCACTGCGTCACCCTGTACCACAATGGTTTTGCCTGCGAAGCCGATACCCTTGGCAGTTGCGGCTACGTATACATCGC
TGTTTATCCCAGTCAACGATAA