Detailed information of TA system TA231978

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsdAT/-
• Location: 1458359..1458873
• Replicon: chromosome
• Accession: NZ_CP090873
• Organism: Staphylococcus aureus strain SAUR_BFS16

Toxin (Protein)

• Gene name: TsdT
• Uniprot ID: T1YA99
• Locus tag: L1A02_RS07070
• Protein ID: WP_000581792.1
• Coordinates: 1458664..1458873 (+)
• Length: 70 a.a.

Antitoxin (Protein)

• Gene name: TsdA
• Uniprot ID: W8UW13
• Locus tag: L1A02_RS07065
• Protein ID: WP_000138932.1
• Coordinates: 1458359..1458652 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L1A02_RS07045 (1453522)	1453522..1454163	+	642	WP_000483153.1	thiamine phosphate synthase	-
L1A02_RS07050 (1454249)	1454249..1455121	+	873	WP_000725802.1	membrane protein insertase YidC	-
L1A02_RS07055 (1456034)	1456034..1456681	-	648	WP_001187630.1	HD domain-containing protein	-
L1A02_RS07060 (1456709)	1456709..1458193	-	1485	WP_000571549.1	cardiolipin synthase	-
L1A02_RS07065 (1458359)	1458359..1458652	+	294	WP_000138932.1	copper-sensing transcriptional repressor CsoR	Antitoxin
L1A02_RS07070 (1458664)	1458664..1458873	+	210	WP_000581792.1	heavy metal-associated domain-containing protein	Toxin
L1A02_RS07075 (1459001)	1459001..1459138	+	138	WP_000828354.1	Lmo0850 family protein	-
L1A02_RS07080 (1459402)	1459402..1460604	-	1203	WP_001109939.1	FtsW/RodA/SpoVE family cell cycle protein	-
L1A02_RS07085 (1460922)	1460922..1461992	+	1071	WP_000159631.1	D-alanine--D-alanine ligase	-
L1A02_RS07090 (1462007)	1462007..1463365	+	1359	WP_000611464.1	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Heavy metal-associated domain, HMA (23-66)

Antitoxin

• Metal-sensitive transcriptional repressor (14-92)

Sequences

Toxin

Protein

• Length: 70 a.a.
• Molecular weight: 7843.77 Da
• Isoelectric Point: 4.1008

>T231978 WP_000581792.1 NZ_CP090873:1458664-1458873 [Staphylococcus aureus]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Nucleotide

• Length: 210 bp

>T231978 NZ_CP127096:1972523-1972711 [Staphylococcus haemolyticus]
ATGAGTTTGCATTTAACTATATTGTTATGGCTTGGTATCATTTTTGTAATAGCAGCCTCAATTATATTGGGCTTATTACT
TAAATCTAAAAAAGAAGAACGTAAAGAATCATATTTAGGTTTTACCGTAATATTTTATATTTTTGGATTTGCTTTACTCA
TCTACGTATTCATATTTGGTATATTATAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11197.82 Da
• Isoelectric Point: 6.2243

>AT231978 WP_000138932.1 NZ_CP090873:1458359-1458652 [Staphylococcus aureus]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Nucleotide

• Length: 294 bp

>AT231978 NZ_CP127096:1972243-1972500 [Staphylococcus haemolyticus]
ATGAATAATAATAAAATTTTTTGGATAAATATTTTTATTACGTTATTATTTTTGGGTTTCAATATCATTGTCACTTACAA
TGCAGGCTTAGATGATTTCTTCTGGCTAATTCCAGGACTTACAATTAGTGGTATTACAATTGTACTAAGCTTAAGTACTG
CGCTCATTTGCAAGAACCTGGTTAGCGAAGTTATTTTTCTAATCAATATTGTTATGCTTTTATACTACATTTATCCTATG
GTATATACATTCTTTTAA

Structures

Toxin

• PDB: 6FF1

Antitoxin

• AlphaFold DB: W8UW13