Detailed information of TA system TA231971

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsaAT/-
• Location: 2099698..2100175
• Replicon: chromosome
• Accession: NZ_CP090870
• Organism: Staphylococcus aureus strain SAUR_BFS62

Toxin (Protein)

• Gene name: TsaT
• Uniprot ID: T1Y7S2
• Locus tag: L0999_RS10700
• Protein ID: WP_000052076.1
• Coordinates: 2099698..2099886 (-)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: TsaA
• Uniprot ID: T1Y6P8
• Locus tag: L0999_RS10705
• Protein ID: WP_001059690.1
• Coordinates: 2099915..2100175 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0999_RS10670 (2095692)	2095692..2096213	+	522	WP_234863250.1	hypothetical protein	-
L0999_RS10675 (2096554)	2096554..2097240	+	687	WP_000141557.1	thermonuclease family protein	-
L0999_RS10680 (2097597)	2097597..2097797	+	201	WP_001059082.1	cold-shock protein	-
L0999_RS10685 (2098289)	2098289..2098507	-	219	WP_000005886.1	hypothetical protein	-
L0999_RS10690 (2098569)	2098569..2098853	-	285	WP_001004399.1	hypothetical protein	-
L0999_RS10695 (2098941)	2098941..2099510	-	570	WP_001106654.1	lipoprotein N-acylation protein LnsA	-
L0999_RS10700 (2099698)	2099698..2099886	-	189	WP_000052076.1	hypothetical protein	Toxin
L0999_RS10705 (2099915)	2099915..2100175	-	261	WP_001059690.1	hypothetical protein	Antitoxin
L0999_RS10710 (2100516)	2100516..2100719	+	204	WP_000145163.1	sterile alpha motif-like domain-containing protein	-
L0999_RS10715 (2100716)	2100716..2100952	-	237	WP_000349581.1	hypothetical protein	-
L0999_RS10720 (2101177)	2101177..2101758	+	582	WP_001140693.1	histidine phosphatase family protein	-
L0999_RS10725 (2101831)	2101831..2102448	-	618	WP_000255551.1	LysE/ArgO family amino acid transporter	-
L0999_RS10730 (2102603)	2102603..2103097	-	495	WP_000569884.1	GNAT family N-acetyltransferase	-
L0999_RS10735 (2103496)	2103496..2103918	-	423	WP_000974460.1	organic hydroperoxide resistance protein	-
L0999_RS10740 (2104066)	2104066..2104782	+	717	WP_234863249.1	type I 3-dehydroquinate dehydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Heavy metal-associated domain, HMA (21-64)

Antitoxin

• Metal-sensitive transcriptional repressor (12-90)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7142.82 Da
• Isoelectric Point: 9.9971

>T231971 WP_000052076.1 NZ_CP090870:c2099886-2099698 [Staphylococcus aureus]
MSLHFAILFWLALIFLVAATFILVLMKKTGKESKKESYLSFTVILYIFGFAILIYTFIFGVL

Nucleotide

• Length: 189 bp

>T231971 NZ_CP127094:927458-927679 [Staphylococcus pseudintermedius]
ATGGAAAATGGTTATGTTAAAGTAACACAACTGACAACTGAAGCACAGCAACAACAGCTATCTGATCGTTTGCTTCGTTT
AATTGGTGTCGAGTCTGTTGCTATTGATGTGGCAACACAATCGTTGACGCTCACTTATGACACGCCAGCAAATTTGAACA
CGATAGAAAAAGAAATTTATGATGCAGGATTTCCTGTTATCCAATCACATAAAGGAGTGTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9940.09 Da
• Isoelectric Point: 4.0624

>AT231971 WP_001059690.1 NZ_CP090870:c2100175-2099915 [Staphylococcus aureus]
MNNILLNAINIVITTTFVIFNILITYNKDLDDLCWLLPGIIICGVILIVSFTIAMITKNWLSEILFFINIVLVLYYIYPI
FYSFIG

Nucleotide

• Length: 261 bp

>AT231971 NZ_CP127094:927158-927445 [Staphylococcus pseudintermedius]
ATGACTGAACAAGCACATCATTCTGAAGAAATTAAACGTAACTTGAAGTCGCGTTTGAATCGTATTGAAGGCCAAGTGAA
AGCAATCAACCGAATGATTGACGAAGATGTCTATTGTGATGACGTGTTGACGCAAATTAGAGCGACACGTTCGGCTTTAA
ACAGTGTGGCGTCGCGTCTGTTAGATCATCATATGAAAGGATGCATCAAGCATAAGATTGACGCGGGACACCAAGACGAA
GCGATGGAAGAGTTATTAGTAACATTTCAAAAACTCATGAAAGATTAG

Structures

Toxin

• AlphaFold DB: A0A0E0VP18

Antitoxin

• AlphaFold DB: A0A0E0VN56