Detailed information of TA system TA231965

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 564120..564649
• Replicon: chromosome
• Accession: NZ_CP090870
• Organism: Staphylococcus aureus strain SAUR_BFS62

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: L0999_RS03150
• Protein ID: WP_000621175.1
• Coordinates: 564120..564482 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: L0999_RS03155
• Protein ID: WP_000948331.1
• Coordinates: 564479..564649 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0999_RS03130 (561098)	561098..561868	-	771	WP_001041111.1	RNA polymerase sigma factor SigB	-
L0999_RS03135 (561843)	561843..562322	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
L0999_RS03140 (562324)	562324..562650	-	327	WP_001052491.1	anti-sigma factor antagonist	-
L0999_RS03145 (562769)	562769..563770	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
L0999_RS03150 (564120)	564120..564482	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
L0999_RS03155 (564479)	564479..564649	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
L0999_RS03160 (564734)	564734..565882	-	1149	WP_234863226.1	alanine racemase	-
L0999_RS03165 (565948)	565948..566307	-	360	WP_000581197.1	holo-ACP synthase	-
L0999_RS03170 (566311)	566311..566802	-	492	WP_001205912.1	PH domain-containing protein	-
L0999_RS03175 (566789)	566789..568372	-	1584	WP_001294626.1	PH domain-containing protein	-
L0999_RS03180 (568365)	568365..568844	-	480	WP_001287087.1	hypothetical protein	-
L0999_RS03185 (569052)	569052..569612	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T231965 WP_000621175.1 NZ_CP090870:c564482-564120 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T231965 NZ_CP127045:c624616-624314 [Pseudomonas amygdali pv. lachrymans]
ATGAATCGCTTTGAACAGACGCCCGCTTTTGCGGAGTGGTTAAGGTCGTTGAAGGACTCCATTGGCAGAGCTCGAATTCT
GGCTCGAATAAGAGCAGCAGAGCTCGGTAACTTCGGGGACTGCGATGCTTTAGGTCAAGGCGTCAGAGAGCTACGAATAC
ACCATGGACCAGGTTATCGGGTGTATTTTGCCCGACGCACGGGTGTGGTTTACCTGCTCTTGATCGCTGGTGACAAATCG
TCTCAAAAGCGCGACATAAAATTTGCCAGACAGCTGGCGAGAGAGCTCTGTGAGAGGGAGTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT231965 WP_000948331.1 NZ_CP090870:c564649-564479 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT231965 NZ_CP127045:c624311-624021 [Pseudomonas amygdali pv. lachrymans]
ATGAACAACGAGACGACACCGTTCGATACCGTCGACTATTTGAAGACACCTGAAGATATGGCCAATTATCTGGATGCGTG
TCTTGAAGAGGATTCAGGAGACGGGCTGCTCGTAAGGGCCGCGCTGAACGACATAGCAAGGGCTTATGGCATGACACAAA
TCGCACGTGATACAGGTCTTGGTCGTGAGAGCCTCTACAAAGCGCTGGGTGCAAACGGTAATCCAGAGTTCACGACCATC
ATGAAAGTCGTGAGGGCGATGGGGATCAAACTACACGCAAGTACGCGCTAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1