Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | PhRel2-ATphRel2/DUF4065(antitoxin) |
| Location | 3339658..3341108 | Replicon | chromosome |
| Accession | NZ_CP090838 | ||
| Organism | Bacillus amyloliquefaciens strain TL106 | ||
Toxin (Protein)
| Gene name | phRel2 | Uniprot ID | - |
| Locus tag | LT978_RS16395 | Protein ID | WP_014304519.1 |
| Coordinates | 3340419..3341108 (-) | Length | 230 a.a. |
Antitoxin (Protein)
| Gene name | ATphRel2 | Uniprot ID | - |
| Locus tag | LT978_RS16390 | Protein ID | WP_014304520.1 |
| Coordinates | 3339658..3340407 (-) | Length | 250 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LT978_RS16355 (LT978_16350) | 3335311..3335616 | - | 306 | WP_014304473.1 | hypothetical protein | - |
| LT978_RS16360 (LT978_16355) | 3335629..3335871 | - | 243 | WP_041481827.1 | helix-turn-helix transcriptional regulator | - |
| LT978_RS16365 (LT978_16360) | 3336041..3336421 | + | 381 | WP_013351190.1 | helix-turn-helix domain-containing protein | - |
| LT978_RS16370 (LT978_16365) | 3336710..3337270 | + | 561 | WP_053574359.1 | DUF4352 domain-containing protein | - |
| LT978_RS16375 (LT978_16370) | 3337454..3337927 | + | 474 | WP_041481826.1 | ImmA/IrrE family metallo-endopeptidase | - |
| LT978_RS16380 (LT978_16375) | 3337939..3339150 | + | 1212 | WP_139890492.1 | site-specific integrase | - |
| LT978_RS16385 (LT978_16380) | 3339435..3339623 | + | 189 | WP_041481838.1 | hypothetical protein | - |
| LT978_RS16390 (LT978_16385) | 3339658..3340407 | - | 750 | WP_014304520.1 | DUF4065 domain-containing protein | Antitoxin |
| LT978_RS16395 (LT978_16390) | 3340419..3341108 | - | 690 | WP_014304519.1 | hypothetical protein | Toxin |
| LT978_RS16400 (LT978_16395) | 3341433..3342404 | - | 972 | WP_252616862.1 | N-acetylmuramoyl-L-alanine amidase | - |
| LT978_RS16405 (LT978_16400) | 3342451..3342873 | - | 423 | WP_014304517.1 | phage holin family protein | - |
| LT978_RS16410 (LT978_16405) | 3342908..3343129 | - | 222 | WP_252616864.1 | hypothetical protein | - |
| LT978_RS16415 (LT978_16410) | 3343143..3343310 | - | 168 | WP_014471524.1 | XkdX family protein | - |
| LT978_RS16420 (LT978_16415) | 3343329..3343616 | - | 288 | WP_119027021.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Prophage | - | groEL | 3290163..3382935 | 92772 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 230 a.a. Molecular weight: 27501.42 Da Isoelectric Point: 8.1662
>T231955 WP_014304519.1 NZ_CP090838:c3341108-3340419 [Bacillus amyloliquefaciens]
MIKCTCRIESFCNHLHQLILEINQLHSDFSTNFSLKQDVKHVHLKKTQIRHVTEVTEKRNIFSIDNDFFILLQHYADKLE
DIVIYNDLEYLYSEFDLRLRVKQPDSIVNKLKYYRVGKKESGQIALRKCLNDLLGIRITIDSFDHNNKCFQDMCELLKKQ
YKINCMDSSKGDYKAFHFYFYGESTFFFPWELQIWRTDDKEANNRSHQLHKQAYKGWASIYKNSTEVEK
MIKCTCRIESFCNHLHQLILEINQLHSDFSTNFSLKQDVKHVHLKKTQIRHVTEVTEKRNIFSIDNDFFILLQHYADKLE
DIVIYNDLEYLYSEFDLRLRVKQPDSIVNKLKYYRVGKKESGQIALRKCLNDLLGIRITIDSFDHNNKCFQDMCELLKKQ
YKINCMDSSKGDYKAFHFYFYGESTFFFPWELQIWRTDDKEANNRSHQLHKQAYKGWASIYKNSTEVEK
Download Length: 690 bp
>T231955 NZ_CP127024:957401-957694 [Staphylococcus aureus]
ATGAATTGGGAAATTAAAGATTTAATGTGTGACATTGAAGTGATAAAACAAAAAATTAATGATGTAGCTACCAAACATGC
TTGGTTTGTTGAAGATAGATTTGTAAAAAATGAATTAGAAACAAAACGGGAACATATTAATTTTTCTGCTAGCTATTTAG
AACATCGTATACAAAATGAACATACAGTTGAGTTATTACAAGTGTACTTAAAAGAGTTCGGTGAACTTATACAAAAGTTT
CATGAAATAGAAAAAGCGTCACTTCAAGCCGATCAAAGCGAAAGTAACGCATAG
ATGAATTGGGAAATTAAAGATTTAATGTGTGACATTGAAGTGATAAAACAAAAAATTAATGATGTAGCTACCAAACATGC
TTGGTTTGTTGAAGATAGATTTGTAAAAAATGAATTAGAAACAAAACGGGAACATATTAATTTTTCTGCTAGCTATTTAG
AACATCGTATACAAAATGAACATACAGTTGAGTTATTACAAGTGTACTTAAAAGAGTTCGGTGAACTTATACAAAAGTTT
CATGAAATAGAAAAAGCGTCACTTCAAGCCGATCAAAGCGAAAGTAACGCATAG
Antitoxin
Download Length: 250 a.a. Molecular weight: 28795.94 Da Isoelectric Point: 6.4113
>AT231955 WP_014304520.1 NZ_CP090838:c3340407-3339658 [Bacillus amyloliquefaciens]
MAFHFIAIISDFSGGRRMGWHYSSEERLDKNYISDFFERVKEKCGMVQFGIHKLSTDSTSWKSVTEKDSFFDDVYVTEDI
STFIDVISKDKELTAYDVAKFVLSFKPASHLKLQKLLYYIYAEFLLKTGEKLFKEPIVAFKYGPVIESIFYKYKTHGKSE
IDYEEDASIKLRTDDVVITPSFMKILSSEYGIEALGCIIEVLVKYGRYSAFGLVEKTHQDGGPWHRVYKPGQNCVITDEL
ITQYHQHAL
MAFHFIAIISDFSGGRRMGWHYSSEERLDKNYISDFFERVKEKCGMVQFGIHKLSTDSTSWKSVTEKDSFFDDVYVTEDI
STFIDVISKDKELTAYDVAKFVLSFKPASHLKLQKLLYYIYAEFLLKTGEKLFKEPIVAFKYGPVIESIFYKYKTHGKSE
IDYEEDASIKLRTDDVVITPSFMKILSSEYGIEALGCIIEVLVKYGRYSAFGLVEKTHQDGGPWHRVYKPGQNCVITDEL
ITQYHQHAL
Download Length: 750 bp
>AT231955 NZ_CP127024:957189-957398 [Staphylococcus aureus]
ATGAATAATGAACAAAAAGAAGTAATAGAACACGTGGTTTATCAACTTGAGTTAAGTGTCATGAATAACTTGGAAAGTTA
TGAACACACAGAATATGTTAATGGTATTGAAGTGGTTTCAGAGATCAGTCGTGAAAAGCACTTAGAATTGATAATGAAAT
GGTGCGCACAAGAATTAAAGAATAATTTTCAATTAGAGAAAGGAGAATAA
ATGAATAATGAACAAAAAGAAGTAATAGAACACGTGGTTTATCAACTTGAGTTAAGTGTCATGAATAACTTGGAAAGTTA
TGAACACACAGAATATGTTAATGGTATTGAAGTGGTTTCAGAGATCAGTCGTGAAAAGCACTTAGAATTGATAATGAAAT
GGTGCGCACAAGAATTAAAGAATAATTTTCAATTAGAGAAAGGAGAATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|
Antitoxin
| Source | ID | Structure |
|---|