Detailed information of TA system TA231954

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PhRel2-ATphRel2/DUF4065(antitoxin)
• Location: 3297907..3299357
• Replicon: chromosome
• Accession: NZ_CP090838
• Organism: Bacillus amyloliquefaciens strain TL106

Toxin (Protein)

• Gene name: phRel2
• Uniprot ID: -
• Locus tag: LT978_RS16060
• Protein ID: WP_014304519.1
• Coordinates: 3298668..3299357 (-)
• Length: 230 a.a.

Antitoxin (Protein)

• Gene name: ATphRel2
• Uniprot ID: -
• Locus tag: LT978_RS16055
• Protein ID: WP_014304520.1
• Coordinates: 3297907..3298656 (-)
• Length: 250 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LT978_RS16040 (LT978_16035)	3294210..3294782	-	573	WP_236823017.1	hypothetical protein	-
LT978_RS16045 (LT978_16040)	3294867..3297152	-	2286	WP_043866880.1	DUF1906 domain-containing protein	-
LT978_RS16050 (LT978_16045)	3297684..3297872	+	189	WP_041481838.1	hypothetical protein	-
LT978_RS16055 (LT978_16050)	3297907..3298656	-	750	WP_014304520.1	DUF4065 domain-containing protein	Antitoxin
LT978_RS16060 (LT978_16055)	3298668..3299357	-	690	WP_014304519.1	hypothetical protein	Toxin
LT978_RS16065 (LT978_16060)	3299682..3300653	-	972	WP_252616862.1	N-acetylmuramoyl-L-alanine amidase	-
LT978_RS16070 (LT978_16065)	3300700..3301122	-	423	WP_014304517.1	phage holin family protein	-
LT978_RS16075 (LT978_16070)	3301157..3301378	-	222	WP_252616864.1	hypothetical protein	-
LT978_RS16080 (LT978_16075)	3301392..3301559	-	168	WP_014471524.1	XkdX family protein	-
LT978_RS16085 (LT978_16080)	3301578..3301865	-	288	WP_119027021.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	groEL	3290163..3382935	92772

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RelA/SpoT (100-215)

Antitoxin

• Antitoxin SocA-like, Panacea domain (113-225)

Sequences

Toxin

Protein

• Length: 230 a.a.
• Molecular weight: 27501.42 Da
• Isoelectric Point: 8.1662

>T231954 WP_014304519.1 NZ_CP090838:c3299357-3298668 [Bacillus amyloliquefaciens]
MIKCTCRIESFCNHLHQLILEINQLHSDFSTNFSLKQDVKHVHLKKTQIRHVTEVTEKRNIFSIDNDFFILLQHYADKLE
DIVIYNDLEYLYSEFDLRLRVKQPDSIVNKLKYYRVGKKESGQIALRKCLNDLLGIRITIDSFDHNNKCFQDMCELLKKQ
YKINCMDSSKGDYKAFHFYFYGESTFFFPWELQIWRTDDKEANNRSHQLHKQAYKGWASIYKNSTEVEK

Nucleotide

• Length: 690 bp

>T231954 NZ_CP127024:c940260-940072 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTAGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATTTTTGGATTCGCTATATTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin

Protein

• Length: 250 a.a.
• Molecular weight: 28795.94 Da
• Isoelectric Point: 6.4113

>AT231954 WP_014304520.1 NZ_CP090838:c3298656-3297907 [Bacillus amyloliquefaciens]
MAFHFIAIISDFSGGRRMGWHYSSEERLDKNYISDFFERVKEKCGMVQFGIHKLSTDSTSWKSVTEKDSFFDDVYVTEDI
STFIDVISKDKELTAYDVAKFVLSFKPASHLKLQKLLYYIYAEFLLKTGEKLFKEPIVAFKYGPVIESIFYKYKTHGKSE
IDYEEDASIKLRTDDVVITPSFMKILSSEYGIEALGCIIEVLVKYGRYSAFGLVEKTHQDGGPWHRVYKPGQNCVITDEL
ITQYHQHAL

Nucleotide

• Length: 750 bp

>AT231954 NZ_CP127024:c940549-940289 [Staphylococcus aureus]
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATCGTATCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTCTATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAA