Detailed information of TA system TA231953

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 2659195..2660112
• Replicon: chromosome
• Accession: NZ_CP090838
• Organism: Bacillus amyloliquefaciens strain TL106

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: LT978_RS12750
• Protein ID: WP_025851786.1
• Coordinates: 2659195..2659941 (+)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: LT978_RS12755
• Protein ID: WP_003154807.1
• Coordinates: 2659942..2660112 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LT978_RS12730 (LT978_12725)	2654588..2655538	-	951	WP_015388352.1	ring-cleaving dioxygenase	-
LT978_RS12735 (LT978_12730)	2655861..2657177	+	1317	WP_003154801.1	amino acid permease	-
LT978_RS12740 (LT978_12735)	2657463..2658080	+	618	WP_003154804.1	DUF47 domain-containing protein	-
LT978_RS12745 (LT978_12740)	2658093..2659091	+	999	WP_003154805.1	inorganic phosphate transporter	-
LT978_RS12750 (LT978_12745)	2659195..2659941	+	747	WP_025851786.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
LT978_RS12755 (LT978_12750)	2659942..2660112	+	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
LT978_RS12760 (LT978_12755)	2660209..2660334	+	126	WP_003154809.1	hypothetical protein	-
LT978_RS12765 (LT978_12760)	2660370..2661248	-	879	WP_014304858.1	N-acetylmuramoyl-L-alanine amidase	-
LT978_RS12770 (LT978_12765)	2661262..2661525	-	264	WP_003154813.1	phage holin	-
LT978_RS12775 (LT978_12770)	2661539..2661802	-	264	WP_003154815.1	hemolysin XhlA family protein	-
LT978_RS12780 (LT978_12775)	2661854..2662615	-	762	WP_139890281.1	phage portal protein	-
LT978_RS12785 (LT978_12780)	2662672..2662869	-	198	WP_003154819.1	XkdX family protein	-
LT978_RS12790 (LT978_12785)	2662875..2663246	-	372	WP_003154820.1	XkdW family protein	-
LT978_RS12795 (LT978_12790)	2663259..2664890	-	1632	WP_139890282.1	pyocin knob domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2644845..2706947	62102

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29048.53 Da
• Isoelectric Point: 4.7003

>T231953 WP_025851786.1 NZ_CP090838:2659195-2659941 [Bacillus amyloliquefaciens]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVVGAMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYGEKMNVTASL
CEYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQDRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T231953 NZ_CP127021:c2743592-2743326 [Staphylococcus aureus]
ATGAGCAATTACACGGTTAAGATTAAAAATTCAGCGAAATCAGATTTAAAGAAAATAAAACATTCTTATTTAAAGAAGTC
ATTTTTAGAAATTGTTGAGACTTTAAAAAATGATCCGTATAAAATAACACAATCTTTTGAAAAATTAGAGCCTAAATATT
TAGAGCGATATTCAAGAAGAATTAACCATCAGCACAGGGTCGTCTATACCGTAGATGATCGAAATAAAGAAGTATTAATA
CTATCGGCATGGTCACATTATGATTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT231953 WP_003154807.1 NZ_CP090838:2659942-2660112 [Bacillus amyloliquefaciens]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT231953 NZ_CP127021:c2743849-2743592 [Staphylococcus aureus]
ATGATTATCACTAGCCCTACAGAAGCGAGAAAAGATTTTTATCAATTACTAAAAAATGTTAATAATAATCACGAACCAAT
TTATATTAGTGGCAATAATGCCGAAAATAATGCTGTGATTATAGGTTTAGAAGATTGGAAAAGTATACAAGAGACAATAT
ATCTTGAATCTACTGGTACAATGGACAAAGTAAGAGAAAGAGAAAAAGATAATAGTGGTACAACAAATATAGATGATATT
GATTGGGATAATCTTTAA

Structures

Antitoxin

• AlphaFold DB: I2HQ14