Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2500412..2500976 Replicon chromosome
Accession NZ_CP090837
Organism Streptomyces sp. R527F

Toxin (Protein)


Gene name mazF Uniprot ID A0A8B2PCB1
Locus tag LZ559_RS11150 Protein ID WP_030801744.1
Coordinates 2500412..2500759 (-) Length 116 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID W9FME8
Locus tag LZ559_RS11155 Protein ID WP_006124459.1
Coordinates 2500746..2500976 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LZ559_RS11140 (LZ559_11140) 2495746..2499054 + 3309 WP_030801742.1 S8 family serine peptidase -
LZ559_RS11145 (LZ559_11145) 2499310..2500338 + 1029 WP_030801743.1 aspartate-semialdehyde dehydrogenase -
LZ559_RS11150 (LZ559_11150) 2500412..2500759 - 348 WP_030801744.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LZ559_RS11155 (LZ559_11155) 2500746..2500976 - 231 WP_006124459.1 hypothetical protein Antitoxin
LZ559_RS11160 (LZ559_11160) 2501030..2501530 - 501 WP_234876656.1 DUF1203 domain-containing protein -
LZ559_RS11165 (LZ559_11165) 2501656..2504232 + 2577 WP_030801748.1 aminopeptidase N -
LZ559_RS11170 (LZ559_11170) 2504378..2504923 + 546 WP_234876657.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 12760.80 Da        Isoelectric Point: 10.9280

>T231951 WP_030801744.1 NZ_CP090837:c2500759-2500412 [Streptomyces sp. R527F]
MRRGDIYLIDFEPVRGSEANKARPALIVSNDGANATVERLERGVLTVVPLTSNTARVFPFQVLLPADECRLAVDSKAQCE
QVRAVSPQRLKRRIGSVPLRRMADVDAALRRHLAL

Download         Length: 348 bp

>T231951 NZ_CP127021:c2361659-2361450 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGATGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8285.03 Da        Isoelectric Point: 3.9251

>AT231951 WP_006124459.1 NZ_CP090837:c2500976-2500746 [Streptomyces sp. R527F]
MKISVSLPQEDVDFVDAYAAANAAESRSAVIQAAIELLRTSRLESEYEAAFAEWDKSEDAAFWDRATGDGLTDAAR

Download         Length: 231 bp

>AT231951 NZ_CP127021:c2361964-2361671 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B2PCB1

Antitoxin

Source ID Structure
AlphaFold DB A0A1A9D4H7

References