Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 6543..7068 Replicon plasmid pSE11-2
Accession NC_011413
Organism Escherichia coli SE11

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag ECSE_RS25085 Protein ID WP_001159871.1
Coordinates 6763..7068 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag ECSE_RS25080 Protein ID WP_000813630.1
Coordinates 6543..6761 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECSE_RS25055 3434..3913 + 480 WP_000721504.1 hypothetical protein -
ECSE_RS25060 3941..4420 + 480 WP_000734263.1 hypothetical protein -
ECSE_RS29070 4794..4970 + 177 WP_024210470.1 hypothetical protein -
ECSE_RS25070 5101..5691 - 591 WP_000194546.1 hypothetical protein -
ECSE_RS25075 5691..5948 - 258 WP_000343099.1 hypothetical protein -
ECSE_RS25080 6543..6761 + 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
ECSE_RS25085 6763..7068 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
ECSE_RS25090 7069..7875 + 807 WP_000016975.1 site-specific integrase -
ECSE_RS25095 8562..9293 - 732 WP_000504263.1 replication initiation protein -
ECSE_RS25100 9914..11119 + 1206 WP_001401628.1 AAA family ATPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - caf1A / faeC / faeD 1..91158 91158


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T23195 WP_001159871.1 NC_011413:6763-7068 [Escherichia coli SE11]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T23195 NC_011413:6763-7068 [Escherichia coli SE11]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGTCGATATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGCGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCCGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT23195 WP_000813630.1 NC_011413:6543-6761 [Escherichia coli SE11]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT23195 NC_011413:6543-6761 [Escherichia coli SE11]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGGCT
GGTAAGCACCACCCTGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References