Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 5282172..5282982 Replicon chromosome
Accession NZ_CP090834
Organism Klebsiella pneumoniae strain K-28

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag L0U94_RS26030 Protein ID WP_002887280.1
Coordinates 5282172..5282705 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag L0U94_RS26035 Protein ID WP_002887278.1
Coordinates 5282716..5282982 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L0U94_RS26025 (L0U94_26000) 5281003..5282124 + 1122 WP_002887282.1 cupin domain-containing protein -
L0U94_RS26030 (L0U94_26005) 5282172..5282705 - 534 WP_002887280.1 type II toxin-antitoxin system toxin KacT Toxin
L0U94_RS26035 (L0U94_26010) 5282716..5282982 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
L0U94_RS26040 (L0U94_26015) 5283085..5284518 - 1434 WP_002887275.1 Cu(+)/Ag(+) sensor histidine kinase -
L0U94_RS26045 (L0U94_26020) 5284508..5285191 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
L0U94_RS26050 (L0U94_26025) 5285363..5286748 + 1386 WP_002887267.1 efflux transporter outer membrane subunit -
L0U94_RS26055 (L0U94_26030) 5286766..5287110 + 345 WP_002887266.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T231946 WP_002887280.1 NZ_CP090834:c5282705-5282172 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T231946 NZ_CP127021:957219-957512 [Staphylococcus aureus]
ATGAATTGGGAAATTAAAGATTTAATGTGTGACATTGAAGTGATAAAACAAAAAATTAATGATGTAGCTACCAAACATGC
TTGGTTTGTTGAAGATAGATTTGTAAAAAATGAATTAGAAACAAAACGGGAACATATTAATTTTTCTGCTAGCTATTTAG
AACATCGTATACAAAATGAACATACAGTTGAGTTATTACAAGTGTACTTAAAAGAGTTCGGTGAACTTATACAAAAGTTT
CATGAAATAGAAAAAGCGTCACTTCAAGCCGATCAAAGCGAAAGTAACGCATAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT231946 WP_002887278.1 NZ_CP090834:c5282982-5282716 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT231946 NZ_CP127021:957007-957216 [Staphylococcus aureus]
ATGAATAATGAACAAAAAGAAGTAATAGAACACGTGGTTTATCAACTTGAGTTAAGTGTCATGAATAACTTGGAAAGTTA
TGAACACACAGAATATGTTAATGGTATTGAAGTGGTTTCAGAGATCAGTCGTGAAAAGCACTTAGAATTGATAATGAAAT
GGTGCGCACAAGAATTAAAGAATAATTTTCAATTAGAGAAAGGAGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References