Detailed information of TA system TA231915

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 602313..603016
• Replicon: chromosome
• Accession: NZ_CP090796
• Organism: Enterobacter hormaechei strain Y-2

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: A0A8B5ZZ18
• Locus tag: L0Y15_RS02855
• Protein ID: WP_039268718.1
• Coordinates: 602675..603016 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: A0A8B5ZWQ9
• Locus tag: L0Y15_RS02850
• Protein ID: WP_039268717.1
• Coordinates: 602313..602654 (+)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0Y15_RS02815 (L0Y15_02815)	597466..598341	+	876	WP_039268712.1	GTPase family protein	-
L0Y15_RS02820 (L0Y15_02820)	598585..599301	+	717	WP_039268713.1	WYL domain-containing protein	-
L0Y15_RS02825 (L0Y15_02825)	599337..599789	+	453	WP_039269148.1	hypothetical protein	-
L0Y15_RS02830 (L0Y15_02830)	599861..600334	+	474	WP_039268714.1	hypothetical protein	-
L0Y15_RS02835 (L0Y15_02835)	600455..601276	+	822	WP_039268715.1	DUF932 domain-containing protein	-
L0Y15_RS02840 (L0Y15_02840)	601307..601747	+	441	WP_039268716.1	antirestriction protein	-
L0Y15_RS02845 (L0Y15_02845)	601760..602302	+	543	WP_045259572.1	DNA repair protein RadC	-
L0Y15_RS02850 (L0Y15_02850)	602313..602654	+	342	WP_039268717.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
L0Y15_RS02855 (L0Y15_02855)	602675..603016	+	342	WP_039268718.1	TA system toxin CbtA family protein	Toxin
L0Y15_RS02860 (L0Y15_02860)	603271..604278	+	1008	WP_039268719.1	restriction endonuclease	-
L0Y15_RS02865 (L0Y15_02865)	604459..605295	-	837	Protein_550	HNH endonuclease	-
L0Y15_RS02870 (L0Y15_02870)	605595..606062	+	468	WP_022650322.1	SymE family type I addiction module toxin	-
L0Y15_RS02875 (L0Y15_02875)	606120..606491	-	372	WP_022650323.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	fosA	-	558784..610962	52178

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-109)

Antitoxin

• Antitoxin CbeA (13-112)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12772.83 Da
• Isoelectric Point: 9.6548

>T231915 WP_039268718.1 NZ_CP090796:602675-603016 [Enterobacter hormaechei]
MKTLPATTPQAAKLCLLPVAVWQMLLARLLEQHYGLTLNDTPFSDETVIQEHIDAGISLADAVNFLVEKYGLVRIDRRGF
NWQEQSPYLRAVDILRARQATGLLKRNRISAAR

Nucleotide

• Length: 342 bp

>T231915 NZ_CP126994:c2554866-2554561 [Acidisoma sp. PAMC 29798]
ATGGCGCTTGAGACGACGGGATGGGACCCCACCGACCAACTCCGCTCGCCCGAGGATGAGCAGGCCTACATAGAAGCCGC
CTTCGAGGAAGGCGACCCCGACCTCATCGCGGCCGCCATCGGTGACATTGCCCGCGCACGCGGCATGGCGCAGATCGCGC
GCGAAGCCGGTGTCAGCCGAGAGGCGATGTACAAGTCGTTCCGCGCCGGCGGTAACCCTACACTCGATACGGTCGCGCGT
GTCACAAGGGTGTTAGGCCTGCGACTGCTGGTGCGCCCGCAGCCGGAGCAGGATGCGTGTAGGTGA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12729.40 Da
• Isoelectric Point: 6.9460

>AT231915 WP_039268717.1 NZ_CP090796:602313-602654 [Enterobacter hormaechei]
MKSTDSENDSFLKWGLNRNVTPCFGARLVQEGNRLHYLADRVSITGKFSEAECLKLDVAFPHFISQMESMLTTGGMNPRH
AHCVTLYHNGFTCEADTLGSYGYVYIAVYPTQR

Nucleotide

• Length: 342 bp

>AT231915 NZ_CP126994:c2555158-2554856 [Acidisoma sp. PAMC 29798]
ATGATCGACGTCCGGCAGACCTCGACCTTCTCTGACTGGCTCGATGGCGTTCGTGATCGGCCCGCGGGTCAGCGCATCAA
CCTGCGTATCCGACGCGTCTCGCTTGGCAATTTCGGTGATGTGAAACTGGTGGGAGAGGGCGTTTCGGAACTGCGGATCG
ACTACGGCCCAGGCTATCGCATCTATGTCAGGCGACGCGGCCTGACCGTTGTTCTGCTGCTGTGTGGCGGCGACAAATCG
ACGCAGGATCGCGACATCGCGCGCGCAAAGACTCTGGCAAAGGAAGACGACAATGGCGCTTGA

Structures

Toxin

• AlphaFold DB: A0A8B5ZZ18

Antitoxin

• AlphaFold DB: A0A8B5ZWQ9