Detailed information of TA system TA231913

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/FR47-DUF1778
• Location: 134664..135461
• Replicon: plasmid pFY-1.p1
• Accession: NZ_CP090793
• Organism: Enterobacter hormaechei strain FY-1

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: F5S3R9
• Locus tag: L0Y16_RS24800
• Protein ID: WP_006812498.1
• Coordinates: 134664..135188 (-)
• Length: 175 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: F5S3R8
• Locus tag: L0Y16_RS24805
• Protein ID: WP_006812497.1
• Coordinates: 135192..135461 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0Y16_RS24765 (L0Y16_24765)	131053..131466	+	414	WP_265181451.1	hypothetical protein	-
L0Y16_RS24770 (L0Y16_24770)	131500..132620	+	1121	WP_096147877.1	IS3-like element ISSen4 family transposase	-
L0Y16_RS24775 (L0Y16_24775)	132656..133123	+	468	WP_234863358.1	hypothetical protein	-
L0Y16_RS24780 (L0Y16_24780)	133198..133521	+	324	WP_000064173.1	hypothetical protein	-
L0Y16_RS24785 (L0Y16_24785)	133535..134227	+	693	WP_006812500.1	membrane protein	-
L0Y16_RS24790 (L0Y16_24790)	134229..134480	+	252	WP_000147960.1	hypothetical protein	-
L0Y16_RS24795 (L0Y16_24795)	134473..134637	+	165	WP_006812499.1	hypothetical protein	-
L0Y16_RS24800 (L0Y16_24800)	134664..135188	-	525	WP_006812498.1	GNAT family N-acetyltransferase	Toxin
L0Y16_RS24805 (L0Y16_24805)	135192..135461	-	270	WP_006812497.1	DUF1778 domain-containing protein	Antitoxin
L0Y16_RS24810 (L0Y16_24810)	135821..136486	+	666	WP_023315962.1	AAA family ATPase	-
L0Y16_RS24815 (L0Y16_24815)	136492..136845	+	354	WP_160859104.1	hypothetical protein	-
L0Y16_RS24820 (L0Y16_24820)	136887..137684	-	798	WP_080112990.1	DUF2971 domain-containing protein	-
L0Y16_RS24825 (L0Y16_24825)	137948..138673	+	726	WP_006812583.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaTEM-1B	-	1..138733	138733

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (88-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 175 a.a.
• Molecular weight: 19356.13 Da
• Isoelectric Point: 8.9844

>T231913 WP_006812498.1 NZ_CP090793:c135188-134664 [Enterobacter hormaechei]
VANLTIEMFSEEAVYDFSCFDCGETSLNDFLNNRLAQQHSGRILRGYLLLTRDPIPKVMGFYTLSGSCFARNTLPSNTQQ
RKVPYSDAPSVTLGRLAIDKSIQRQGYGETLVAHAMKVVYRASLAVGIYALFVDAKNENAMRFYKQLGFTPLTGDNANSL
FYPTKGIEKLFGGK

Nucleotide

• Length: 525 bp

>T231913 NZ_CP126994:c1855011-1854622 [Acidisoma sp. PAMC 29798]
ATGTTGGATACCAACATCGTCTCTGATCTGATCCGCAACCCAGTCGGCCGGGTCGCGGAGCAACTTTTGCTTCATGGTAA
CGACGGCCTGTGTCTCAGCATCATTACGGCGGCGGAACTGCGTTACGGCGCGGCGAAAAAAGGCTCCGACCGTCTGTCTG
CCCGCGTGAGTGCCGTGCTTGGGACGCTCGATATCCTGCCTTTCGACAGCCCCGCTGATGCCGAATACGGAAAGCTGCGC
GCCGCGCTGGAAGCGGCTGGGACGCCGATCAGGCCGACCGATCTTTTCATCGCCGCACACGCTCTGGCACTGCAGGTGAC
GCTGGTCACCCACAACGTCTCGGAATTTCGTTGGGTACGCGGGCTGACGGTTGAAAACTGGCTGGCTTAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10114.51 Da
• Isoelectric Point: 4.8483

>AT231913 WP_006812497.1 NZ_CP090793:c135461-135192 [Enterobacter hormaechei]
MSALKKQRIDLRLTDDDKSMIEEAAAMTNQTITQFMVASASERAAEVIEQHRRLILSEESWNIVMDAISNPPAPNDRLKR
AAERLQSME

Nucleotide

• Length: 270 bp

>AT231913 NZ_CP126994:c1855265-1855026 [Acidisoma sp. PAMC 29798]
ATGTCCGAAGCGCGACAGGTTCGGTTGTTCCGCAATGGTCGGAATCAGGCTGTCCGCATTCCGGTGGAGTTTGAACTTCC
GGGTGAGGCGGCCCTTATGCGTCGCGAGGGCAATCGGCTTGTGATCGAGCCCATTCGGAAGGGCGGGCTGATCGAATTGC
TTGCCACCTGGGATCCGATCGACGACGATTTCGCGGATTTTGACGAAGGCCTGCAACCCGCCCGAGACATCGATCTGTGA

Structures

Toxin

• AlphaFold DB: A0A2J0QWY8

Antitoxin

• AlphaFold DB: A0A2J0QUA3