Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/ParE-RHH
Location 12669..13204 Replicon plasmid pRLG204
Accession NC_011371
Organism Rhizobium leguminosarum bv. trifolii WSM2304

Toxin (Protein)


Gene name parE Uniprot ID B6A4M0
Locus tag RLEG2_RS30360 Protein ID WP_012559995.1
Coordinates 12669..12959 (-) Length 97 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID B6A4M1
Locus tag RLEG2_RS30365 Protein ID WP_012559996.1
Coordinates 12956..13204 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RLEG2_RS30330 8144..8764 + 621 WP_003595611.1 1-acyl-sn-glycerol-3-phosphate acyltransferase -
RLEG2_RS30335 8761..9690 + 930 WP_012559991.1 phosphatidate cytidylyltransferase -
RLEG2_RS30340 9713..10036 - 324 WP_003595609.1 transcriptional regulator -
RLEG2_RS30345 10039..10953 - 915 WP_012559992.1 J domain-containing protein -
RLEG2_RS30350 11047..11514 - 468 WP_012559993.1 universal stress protein -
RLEG2_RS30355 11727..12593 + 867 WP_012559994.1 NAD(P)-dependent oxidoreductase -
RLEG2_RS30360 12669..12959 - 291 WP_012559995.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
RLEG2_RS30365 12956..13204 - 249 WP_012559996.1 type II toxin-antitoxin system ParD family antitoxin Antitoxin
RLEG2_RS30370 13416..14291 + 876 WP_012559997.1 glutathione-dependent disulfide-bond oxidoreductase -
RLEG2_RS30375 14374..15888 - 1515 WP_012559998.1 ABC transporter substrate-binding protein -
RLEG2_RS30380 15908..17617 - 1710 WP_012559999.1 ABC transporter ATP-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - gmd 1..257956 257956


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin

Antitoxin ParD

(4-71)


Sequences

Toxin        


Download         Length: 97 a.a.        Molecular weight: 11081.58 Da        Isoelectric Point: 8.9401

>T23191 WP_012559995.1 NC_011371:c12959-12669 [Rhizobium leguminosarum bv. trifolii WSM2304]
MTGIIFSPAAQTDIDRIWDYTATSWNVDQAERYIQDIRDACHELAQGTRMSRPSDIRKGYRKVSVGSHFLYFKSNDAGQI
IVVRILHQRMDVAKHL

Download         Length: 291 bp

>T23191 NC_011371:c12959-12669 [Rhizobium leguminosarum bv. trifolii WSM2304]
ATGACAGGGATCATATTTTCCCCCGCCGCGCAGACCGATATTGACAGGATTTGGGATTACACAGCGACAAGTTGGAATGT
CGATCAGGCAGAGCGTTACATTCAGGACATAAGAGACGCCTGTCACGAACTGGCGCAGGGAACACGGATGAGCCGTCCCT
CAGACATTCGGAAAGGGTACAGAAAGGTCTCCGTTGGCTCTCATTTTCTGTATTTCAAAAGTAATGATGCAGGCCAGATC
ATCGTTGTACGCATCCTTCATCAAAGAATGGATGTAGCCAAACACTTGTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9001.02 Da        Isoelectric Point: 5.0760

>AT23191 WP_012559996.1 NC_011371:c13204-12956 [Rhizobium leguminosarum bv. trifolii WSM2304]
MARNTSISLSDHFVSFIDTQVQAGRYGSATDVVRAGLRLLEEHEAKVKALQDALIEGEESGPATPFDFDAFNARKRATFE
AK

Download         Length: 249 bp

>AT23191 NC_011371:c13204-12956 [Rhizobium leguminosarum bv. trifolii WSM2304]
ATGGCCAGAAATACGTCCATATCGCTCAGCGACCATTTCGTCAGCTTCATCGATACGCAAGTTCAGGCTGGCCGCTATGG
TTCAGCCACTGACGTCGTTCGAGCCGGCCTTCGCCTCCTTGAAGAGCACGAAGCCAAAGTGAAGGCATTACAGGACGCCT
TGATAGAAGGTGAGGAATCCGGCCCTGCCACACCCTTCGATTTCGATGCGTTCAACGCTCGCAAACGGGCGACGTTCGAG
GCGAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7Z0E3H3

Antitoxin

Source ID Structure
AlphaFold DB A0A1W6KTA2

References