Detailed information of TA system TA023190

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 4472667..4473469
• Replicon: chromosome
• Accession: NC_011369
• Organism: Rhizobium leguminosarum bv. trifolii WSM2304

Toxin (Protein)

• Gene name: TacT3
• Uniprot ID: B5ZYE8
• Locus tag: RLEG2_RS21680
• Protein ID: WP_012559676.1
• Coordinates: 4472942..4473469 (+)
• Length: 176 a.a.

Antitoxin (Protein)

• Gene name: TacA3
• Uniprot ID: B5ZYE7
• Locus tag: RLEG2_RS21675
• Protein ID: WP_012559675.1
• Coordinates: 4472667..4472945 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RLEG2_RS21645	4468011..4469021	+	1011	WP_012559669.1	helix-turn-helix domain-containing protein	-
RLEG2_RS21650	4469179..4470033	+	855	WP_012559670.1	helix-turn-helix transcriptional regulator	-
RLEG2_RS21655	4470072..4470617	+	546	WP_012559671.1	YbhB/YbcL family Raf kinase inhibitor-like protein	-
RLEG2_RS21660	4470643..4471269	-	627	WP_012559672.1	dihydrofolate reductase family protein	-
RLEG2_RS21665	4471359..4471757	-	399	WP_012559673.1	VOC family protein	-
RLEG2_RS21670	4471855..4472556	+	702	WP_012559674.1	YafY family transcriptional regulator	-
RLEG2_RS21675	4472667..4472945	+	279	WP_012559675.1	DUF1778 domain-containing protein	Antitoxin
RLEG2_RS21680	4472942..4473469	+	528	WP_012559676.1	hypothetical protein	Toxin
RLEG2_RS21685	4473630..4475192	-	1563	WP_012559677.1	glutamine-hydrolyzing GMP synthase	-
RLEG2_RS21690	4475305..4475943	-	639	WP_003589098.1	5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase	-
RLEG2_RS21695	4475940..4476389	-	450	WP_003544704.1	PaaI family thioesterase	-
RLEG2_RS21700	4476621..4477697	+	1077	WP_012559678.1	DUF2252 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Vibrio phage ICP1, Orf50 (11-87)

Sequences

Toxin

Protein

• Length: 176 a.a.
• Molecular weight: 18738.44 Da
• Isoelectric Point: 7.3519

>T23190 WP_012559676.1 NC_011369:4472942-4473469 [Rhizobium leguminosarum bv. trifolii WSM2304]
MTLPAWREEAIAKSHDRQSFDCGDLAMNDFFRRYARQSHEQNASKTFCAIDASTPNRVLGFYTVAPSSVAHEGVPASMTK
GLAQHEVPGFKLARIATDISVAGHGLGGQLLAAAALRCLRIASEAGGVLLIIDAKGERAPQWYVSYGAEPLENQPLTLVM
PLATFAADLKGKGLL

Nucleotide

• Length: 528 bp

>T23190 NC_011369:4472942-4473469 [Rhizobium leguminosarum bv. trifolii WSM2304]
ATGACGCTGCCCGCCTGGAGAGAGGAAGCCATCGCGAAATCGCATGATCGCCAGTCCTTCGACTGCGGCGATCTGGCGAT
GAACGACTTTTTCCGACGCTACGCGCGTCAAAGCCACGAACAGAACGCCTCAAAGACCTTTTGCGCGATCGACGCCTCTA
CGCCCAACCGTGTGCTGGGGTTCTACACTGTCGCCCCCTCCTCTGTGGCACATGAAGGTGTGCCGGCGTCGATGACGAAG
GGGCTGGCCCAGCACGAAGTTCCTGGGTTCAAGCTGGCTCGGATCGCAACCGATATCTCTGTAGCGGGCCATGGTTTGGG
TGGACAGCTCCTCGCGGCCGCGGCGCTTCGCTGCCTGCGGATCGCGTCTGAAGCAGGCGGCGTTCTGCTGATTATCGACG
CAAAGGGCGAGCGCGCGCCCCAATGGTATGTCAGCTATGGGGCCGAGCCGCTGGAGAACCAGCCGCTTACGCTTGTCATG
CCGCTCGCCACCTTCGCCGCTGATCTCAAGGGCAAGGGGCTTCTCTGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10093.69 Da
• Isoelectric Point: 10.5807

>AT23190 WP_012559675.1 NC_011369:4472667-4472945 [Rhizobium leguminosarum bv. trifolii WSM2304]
MPRAAVDDNKRMNLRVSPETKAKLVRAAALRNTDLTNFVTQTALREAEAVIEAAEVVRLTARDHARVLELLENPPKPNAK
LRAAIAALPDTL

Nucleotide

• Length: 279 bp

>AT23190 NC_011369:4472667-4472945 [Rhizobium leguminosarum bv. trifolii WSM2304]
ATGCCCCGAGCTGCCGTAGATGACAACAAGCGCATGAATCTGCGGGTCTCCCCGGAAACGAAGGCCAAGCTCGTGCGCGC
CGCTGCGCTGCGCAACACCGATTTGACCAACTTCGTGACCCAGACCGCCTTGCGCGAAGCCGAAGCCGTGATCGAGGCGG
CCGAGGTCGTTCGCCTGACGGCGCGCGATCATGCGCGCGTGCTGGAACTGCTGGAAAATCCGCCCAAGCCCAACGCGAAG
CTGCGGGCGGCGATCGCGGCGTTGCCCGATACTCTATGA

Structures

Toxin

• AlphaFold DB: B5ZYE8

Antitoxin

• AlphaFold DB: B5ZYE7