Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 1822616..1823413 Replicon chromosome
Accession NZ_CP090792
Organism Enterobacter hormaechei strain FY-1

Toxin (Protein)


Gene name KacT Uniprot ID A0A222RDD1
Locus tag L0Y16_RS08680 Protein ID WP_032647926.1
Coordinates 1822892..1823413 (+) Length 174 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID F5RT92
Locus tag L0Y16_RS08675 Protein ID WP_001303459.1
Coordinates 1822616..1822885 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L0Y16_RS08640 (L0Y16_08640) 1818330..1819211 + 882 WP_032653182.1 DUF6402 family protein -
L0Y16_RS08645 (L0Y16_08645) 1819208..1819639 + 432 WP_050597034.1 DUF6201 family protein -
L0Y16_RS08650 (L0Y16_08650) 1819792..1819893 + 102 Protein_1681 type VI secretion system lipoprotein TssJ -
L0Y16_RS08655 (L0Y16_08655) 1820117..1820344 - 228 WP_006809324.1 YccJ family protein -
L0Y16_RS08660 (L0Y16_08660) 1820363..1820959 - 597 WP_006809323.1 NAD(P)H:quinone oxidoreductase -
L0Y16_RS08665 (L0Y16_08665) 1821350..1821520 + 171 WP_001273664.1 general stress protein -
L0Y16_RS08670 (L0Y16_08670) 1821637..1822542 + 906 WP_032653183.1 DMT family transporter -
L0Y16_RS08675 (L0Y16_08675) 1822616..1822885 + 270 WP_001303459.1 DUF1778 domain-containing protein Antitoxin
L0Y16_RS08680 (L0Y16_08680) 1822892..1823413 + 522 WP_032647926.1 GNAT family N-acetyltransferase Toxin
L0Y16_RS08685 (L0Y16_08685) 1823472..1824794 - 1323 WP_032653185.1 pyrimidine utilization transport protein G -
L0Y16_RS08690 (L0Y16_08690) 1824816..1825307 - 492 WP_032653187.1 pyrimidine utilization flavin reductase protein F -
L0Y16_RS08695 (L0Y16_08695) 1825320..1825910 - 591 WP_017693662.1 malonic semialdehyde reductase -
L0Y16_RS08700 (L0Y16_08700) 1825920..1826720 - 801 WP_023296287.1 pyrimidine utilization protein D -
L0Y16_RS08705 (L0Y16_08705) 1826728..1827114 - 387 WP_015570870.1 pyrimidine utilization protein C -
L0Y16_RS08710 (L0Y16_08710) 1827126..1827815 - 690 WP_032653188.1 pyrimidine utilization protein B -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(87-149)

Antitoxin

Vibrio phage ICP1, Orf50

(2-88)


Sequences

Toxin        


Download         Length: 174 a.a.        Molecular weight: 19451.21 Da        Isoelectric Point: 7.4318

>T231898 WP_032647926.1 NZ_CP090792:1822892-1823413 [Enterobacter hormaechei]
VANLTIEMLSEGTDYDFGDFDCGEPSLNAFLTEHLIRQHGGRILRGYLLKERDHPRVLGYYTLSGSCFEKAMLPSKTQQR
RIPYSNVPSVTLGRLAVHKELQGNEWGTTLVTHAMRVVYLASQAVGVHGIFVDALNERAKRFYLKLGFIPLAGENSSSLF
FPTQSIERLFEQA

Download         Length: 522 bp

>T231898 NZ_CP126991:c7296-6982 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCCACAGGTAACGATTTCCGCGCTGGCACAGCGCGATTTACAACGCCTTCAGGACTTTCTCAAAACCAAAAATCGGCT
GGCAGCCAGAAAAGGAGGTGAGGTGATCGTCCGGGCTATCCAGCAACTGAAAACATTGCCAGACATTGGCCGCCCGGTGC
CGTTTCTGCCGCTGGAATATAAGGAACTGGTGATCGGGTTTGGCGACAGTGGCTATGTGATGCTCTACCGCCACGACCGG
GAAATGGACCAGATTGTGATCGTCACCGTCAGGCATCAGAAAGAATCCGGGTATCCGGGGGCAGACAGCCTCTAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10248.70 Da        Isoelectric Point: 5.8836

>AT231898 WP_001303459.1 NZ_CP090792:1822616-1822885 [Enterobacter hormaechei]
MPALKKQRIDLRLSDEDKTMIEEAAAMTNQTITQFMVNSASERAAEVIEQHRRLVLNEASWNAVMDAIDNPPEPNERLKR
AAKRLRNME

Download         Length: 270 bp

>AT231898 NZ_CP126991:c7562-7284 [Klebsiella pneumoniae subsp. pneumoniae]
ATGTCATCAGCAACATCCATCAGGCTCGATGAAGAACTTAAGGACCGGCTGAAAACGCTGGCCGATGACCGGCACCGCTC
AGCGCACGCGCTAATGCTGGAGGCAATCACCGAATACATCGACCGGGAAGAGAAGCGCAGCCAGTACCTGCGGGACGGAC
AGGCCGCATGGCAGCACTATCAGGAAACGGGTCTGCACCTTACTGCGGAAGAAGCGGAAGCCTGGATAAGTACCTGGGGA
ACTGAGAACGAGCAGGATGCTCCGCCATGCCACAGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A222RDD1

Antitoxin

Source ID Structure
AlphaFold DB A0A7D7BCZ2

References