Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK-RelB
Location 220076..220631 Replicon chromosome
Accession NZ_CP090790
Organism Bifidobacterium longum strain D2TC

Toxin (Protein)


Gene name mazF Uniprot ID B7GSS5
Locus tag LMY38_RS01100 Protein ID WP_003833460.1
Coordinates 220308..220631 (+) Length 108 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID D6ZTS2
Locus tag LMY38_RS01095 Protein ID WP_013140588.1
Coordinates 220076..220321 (+) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LMY38_RS01075 (LMY38_01075) 216941..217603 + 663 WP_019728044.1 TetR/AcrR family transcriptional regulator -
LMY38_RS01080 (LMY38_01080) 217726..217953 + 228 Protein_216 MFS transporter -
LMY38_RS01085 (LMY38_01085) 218190..218546 - 357 WP_231938191.1 hypothetical protein -
LMY38_RS01090 (LMY38_01090) 218646..219902 - 1257 WP_003829116.1 HipA domain-containing protein -
LMY38_RS01095 (LMY38_01095) 220076..220321 + 246 WP_013140588.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
LMY38_RS01100 (LMY38_01100) 220308..220631 + 324 WP_003833460.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LMY38_RS01105 (LMY38_01105) 220848..221462 - 615 WP_080770920.1 cupin domain-containing protein -
LMY38_RS01110 (LMY38_01110) 221610..221969 - 360 WP_080770921.1 class I SAM-dependent methyltransferase -
LMY38_RS01115 (LMY38_01115) 222207..222572 + 366 WP_013140585.1 YccF domain-containing protein -
LMY38_RS01120 (LMY38_01120) 223114..224379 + 1266 WP_050494278.1 Fic family protein -
LMY38_RS01125 (LMY38_01125) 224535..225629 - 1095 WP_032683870.1 redox-regulated ATPase YchF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-106)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(5-56)


Sequences

Toxin        


Download         Length: 108 a.a.        Molecular weight: 11829.64 Da        Isoelectric Point: 6.2932

>T231893 WP_003833460.1 NZ_CP090790:220308-220631 [Bifidobacterium longum]
MKRGEIRTLQADGYASKPRPVVIVQSDAVDRFDSVITCLLTSYDSSDIDTRVRLEPSPENGLNKVSYVMTDKIVTVDRKL
LGYQVGVVDDAAMANIGRQLMRVLGLL

Download         Length: 324 bp

>T231893 NZ_CP126987:4004636-4004854 [Klebsiella pneumoniae subsp. pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9293.39 Da        Isoelectric Point: 4.7719

>AT231893 WP_013140588.1 NZ_CP090790:220076-220321 [Bifidobacterium longum]
MSIQIATRVSDEQAALFKETTRQLGTTPADALRMFISAFNDYRGFPYEVRLPRNDVEPFASERDATEYASRLALRMSDET
R

Download         Length: 246 bp

>AT231893 NZ_CP126987:4004235-4004609 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4R0SL31

Antitoxin

Source ID Structure
AlphaFold DB A0A087BFX1

References