Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 4234111..4234700 Replicon chromosome
Accession NC_011369
Organism Rhizobium leguminosarum bv. trifolii WSM2304

Toxin (Protein)


Gene name hicA Uniprot ID B5ZW21
Locus tag RLEG2_RS20445 Protein ID WP_012559482.1
Coordinates 4234111..4234308 (+) Length 66 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID B5ZW22
Locus tag RLEG2_RS20450 Protein ID WP_012559483.1
Coordinates 4234305..4234700 (+) Length 132 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RLEG2_RS20415 4229389..4229862 - 474 WP_012559479.1 hypothetical protein -
RLEG2_RS20420 4230038..4230673 + 636 WP_012559480.1 trimeric intracellular cation channel family protein -
RLEG2_RS20425 4230686..4231105 - 420 WP_003555773.1 transcriptional repressor -
RLEG2_RS20430 4231406..4231921 + 516 WP_003589358.1 3-hydroxyacyl-[acyl-carrier-protein] dehydratase FabA -
RLEG2_RS20435 4231965..4233197 + 1233 WP_012559481.1 beta-ketoacyl-ACP synthase I -
RLEG2_RS20440 4233202..4234008 + 807 WP_003589356.1 enoyl-ACP reductase FabI -
RLEG2_RS20445 4234111..4234308 + 198 WP_012559482.1 type II toxin-antitoxin system HicA family toxin Toxin
RLEG2_RS20450 4234305..4234700 + 396 WP_012559483.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
RLEG2_RS20455 4234704..4235720 - 1017 WP_012559484.1 class I SAM-dependent methyltransferase -
RLEG2_RS20460 4235850..4237988 - 2139 WP_003589352.1 polyribonucleotide nucleotidyltransferase -
RLEG2_RS20465 4238345..4238614 - 270 WP_003571238.1 30S ribosomal protein S15 -
RLEG2_RS20470 4238768..4239619 - 852 WP_012559485.1 alpha/beta hydrolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(6-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(4-124)


Sequences

Toxin        


Download         Length: 66 a.a.        Molecular weight: 7209.38 Da        Isoelectric Point: 11.4650

>T23189 WP_012559482.1 NC_011369:4234111-4234308 [Rhizobium leguminosarum bv. trifolii WSM2304]
VERNSQKIIARLKREGFEIVSIKGSHHKLRRGNQTIIVPHPKKDLPIGTALAIAKQAGWNASDKS

Download         Length: 198 bp

>T23189 NC_011369:4234111-4234308 [Rhizobium leguminosarum bv. trifolii WSM2304]
GTGGAGCGGAACAGTCAGAAGATTATCGCCAGGCTGAAGCGGGAAGGCTTCGAAATCGTTTCGATCAAGGGCTCGCACCA
CAAGCTTCGAAGAGGCAATCAGACCATCATCGTTCCGCACCCGAAGAAGGATCTGCCGATCGGCACGGCGCTTGCTATTG
CTAAGCAAGCCGGATGGAATGCGAGTGACAAATCATGA

Antitoxin


Download         Length: 132 a.a.        Molecular weight: 14445.23 Da        Isoelectric Point: 4.3257

>AT23189 WP_012559483.1 NC_011369:4234305-4234700 [Rhizobium leguminosarum bv. trifolii WSM2304]
MKYFIALVHKDSDSAFGISFPDLPAVFSAADEEEDLTANAIEALRLWAEDETLPMPSSYDEISSRQDIREQLAAGAFLTR
VPFIEDTTRTVRANVTFDKGMLEAIDKAAKERGLTRSAFLASAARKEIEAA

Download         Length: 396 bp

>AT23189 NC_011369:4234305-4234700 [Rhizobium leguminosarum bv. trifolii WSM2304]
ATGAAATATTTCATTGCTCTCGTGCATAAGGACAGCGACAGCGCATTCGGGATCAGCTTTCCAGATCTGCCGGCGGTATT
TTCGGCCGCCGATGAGGAAGAAGACCTGACTGCCAACGCCATAGAAGCCCTTCGCCTCTGGGCGGAAGATGAAACTTTGC
CGATGCCATCCTCATATGACGAGATCAGTTCTCGGCAGGATATCCGCGAGCAACTGGCTGCCGGTGCTTTCCTGACGCGT
GTTCCCTTTATCGAAGACACCACGCGCACCGTTCGAGCCAATGTCACTTTCGACAAAGGCATGCTTGAGGCAATCGACAA
GGCTGCAAAAGAGCGTGGTCTGACCCGTTCGGCTTTCCTTGCGAGTGCGGCCCGCAAGGAAATCGAAGCCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0N1DMY0

Antitoxin

Source ID Structure
AlphaFold DB B5ZW22

References