Detailed information of TA system TA023188

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 3734355..3734917
• Replicon: chromosome
• Accession: NC_011369
• Organism: Rhizobium leguminosarum bv. trifolii WSM2304

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: B5ZSJ7
• Locus tag: RLEG2_RS18185
• Protein ID: WP_012559155.1
• Coordinates: 3734355..3734603 (+)
• Length: 83 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: B5ZSJ8
• Locus tag: RLEG2_RS18190
• Protein ID: WP_012559156.1
• Coordinates: 3734600..3734917 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RLEG2_RS18160	3730092..3730337	-	246	WP_041671554.1	hypothetical protein	-
RLEG2_RS18165	3730386..3731570	-	1185	WP_012559152.1	GTP-binding protein	-
RLEG2_RS18170	3731594..3732727	-	1134	WP_012559153.1	D-alanyl-D-alanine carboxypeptidase	-
RLEG2_RS18175	3732915..3734078	+	1164	WP_012559154.1	amidohydrolase	-
RLEG2_RS18185	3734355..3734603	+	249	WP_012559155.1	BrnT family toxin	Toxin
RLEG2_RS18190	3734600..3734917	+	318	WP_012559156.1	BrnA antitoxin family protein	Antitoxin
RLEG2_RS18195	3734992..3735510	-	519	WP_012559157.1	DUF2937 family protein	-
RLEG2_RS18200	3735655..3735915	+	261	WP_003589897.1	hypothetical protein	-
RLEG2_RS34925	3736021..3736173	+	153	WP_012559158.1	hypothetical protein	-
RLEG2_RS18205	3736199..3736489	-	291	WP_012559159.1	BON domain-containing protein	-
RLEG2_RS18210	3736681..3737331	-	651	WP_041671773.1	hypothetical protein	-
RLEG2_RS18215	3737693..3738883	+	1191	WP_041671775.1	1-deoxy-D-xylulose-5-phosphate reductoisomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-77)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (27-103)

Sequences

Toxin

Protein

• Length: 83 a.a.
• Molecular weight: 9175.68 Da
• Isoelectric Point: 7.0136

>T23188 WP_012559155.1 NC_011369:3734355-3734603 [Rhizobium leguminosarum bv. trifolii WSM2304]
MEIVWDEPKRIANIEKHGLDFADLTFEFFLSSVVVPAKDGRSKAIGRLADGTIVVIFVNLGSEALSIISMRPARKDERSM
IR

Nucleotide

• Length: 249 bp

>T23188 NC_011369:3734355-3734603 [Rhizobium leguminosarum bv. trifolii WSM2304]
ATGGAGATCGTGTGGGACGAACCGAAGCGGATTGCCAATATCGAGAAACATGGCCTCGATTTCGCCGATCTGACTTTCGA
GTTCTTCCTGTCTTCCGTTGTCGTTCCCGCCAAAGATGGGCGATCCAAGGCCATAGGCCGCCTCGCTGACGGAACGATCG
TAGTGATCTTCGTAAATTTGGGCTCTGAGGCGCTTTCGATCATTTCGATGCGCCCCGCCCGCAAGGACGAAAGGAGCATG
ATCCGATGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11892.66 Da
• Isoelectric Point: 10.4275

>AT23188 WP_012559156.1 NC_011369:3734600-3734917 [Rhizobium leguminosarum bv. trifolii WSM2304]
MIIKARNTTQFQLGRGYSKEDWDAVSDNPPLSKEEMARAKPFKEAFPDVAEKMEKAIAARGRPKLDNPKQPLNIRLDTDI
IQFYKATGKGWQSRMNDALRKAAGL

Nucleotide

• Length: 318 bp

>AT23188 NC_011369:3734600-3734917 [Rhizobium leguminosarum bv. trifolii WSM2304]
ATGATTATCAAAGCCCGGAATACGACCCAGTTCCAGCTTGGCCGCGGCTACTCCAAAGAGGATTGGGATGCCGTCTCCGA
CAATCCCCCTCTGTCCAAGGAAGAGATGGCAAGAGCCAAGCCCTTCAAGGAGGCATTTCCTGACGTTGCTGAAAAGATGG
AGAAGGCCATTGCAGCCCGCGGTCGACCCAAGCTGGACAACCCGAAGCAACCCCTGAACATCCGTCTCGATACCGATATT
ATCCAGTTCTACAAGGCCACTGGCAAAGGCTGGCAGTCAAGGATGAACGATGCATTGCGAAAGGCGGCTGGCTTATAG

Structures

Toxin

• AlphaFold DB: B5ZSJ7

Antitoxin

• AlphaFold DB: B5ZSJ8