Detailed information of TA system TA231867

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 759060..759637
• Replicon: chromosome
• Accession: NZ_CP090776
• Organism: Burkholderia multivorans strain 2006Ycf877

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: L0Z45_RS22840
• Protein ID: WP_053298683.1
• Coordinates: 759060..759425 (-)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A2S9ML45
• Locus tag: L0Z45_RS22845
• Protein ID: WP_035956044.1
• Coordinates: 759419..759637 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0Z45_RS22815 (L0Z45_22815)	754237..755136	+	900	WP_035487807.1	lipid A hydroxylase LpxO	-
L0Z45_RS22820 (L0Z45_22820)	755260..755988	+	729	WP_249475708.1	hypothetical protein	-
L0Z45_RS22825 (L0Z45_22825)	756050..757648	-	1599	WP_053298685.1	alkyl hydroperoxide reductase subunit F	-
L0Z45_RS22830 (L0Z45_22830)	757767..758330	-	564	WP_006399806.1	alkyl hydroperoxide reductase subunit C	-
L0Z45_RS22835 (L0Z45_22835)	758776..758964	-	189	WP_249475710.1	hypothetical protein	-
L0Z45_RS22840 (L0Z45_22840)	759060..759425	-	366	WP_053298683.1	type II toxin-antitoxin system VapC family toxin	Toxin
L0Z45_RS22845 (L0Z45_22845)	759419..759637	-	219	WP_035956044.1	ribbon-helix-helix protein, CopG family	Antitoxin
L0Z45_RS22850 (L0Z45_22850)	759739..760635	-	897	WP_035954582.1	LysR substrate-binding domain-containing protein	-
L0Z45_RS22855 (L0Z45_22855)	760811..761971	+	1161	WP_249475712.1	branched-chain amino acid ABC transporter substrate-binding protein	-
L0Z45_RS22860 (L0Z45_22860)	762139..762678	+	540	WP_053298682.1	L-2-amino-thiazoline-4-carboxylic acid hydrolase	-
L0Z45_RS22865 (L0Z45_22865)	762671..763942	+	1272	WP_006403830.1	Zn-dependent hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-106)

Antitoxin

• Ribbon-helix-helix protein, CopG (6-40)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13556.55 Da
• Isoelectric Point: 5.1515

>T231867 WP_053298683.1 NZ_CP090776:c759425-759060 [Burkholderia multivorans]
MVKALFDTNILIDYLSGVESARLELGRYDHRAISTITWMEVLVGTTADDEGPIRAWLSSFDVIPLDSTVADRAVTIRKER
RIRLPDAIVWASAQVNGLLLVSRNTKDFPSTEPGIRVPYQI

Nucleotide

• Length: 366 bp

>T231867 NZ_CP126975:c2033897-2033715 [Gallibacterium anatis]
GTGGATAGTAAAACATTAATAAAAATGCTTGAAAAAGATGGGTGGTACTTGGTGAATGTGGTTGGTAGTCATCATCAATT
TAAACATCCAATAAAGAAAGGGCGGACAACAGTTCCGCATCCCAAAAAGGATTTACAAATAAAAACGGTAAATTCAATAT
TAAAACAAGCGGGCTTGAAATAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8231.38 Da
• Isoelectric Point: 4.7640

>AT231867 WP_035956044.1 NZ_CP090776:c759637-759419 [Burkholderia multivorans]
MSRVLVDLSDGQLDELAVIVETEHRPRAAIIRDAIDAYIALHKRPLADDVFGLWKDRAVDGLAYQEKLRSEW

Nucleotide

• Length: 219 bp

>AT231867 NZ_CP126975:c2033687-2033280 [Gallibacterium anatis]
ATGTTATATCCGATTGGAATTGAAATGGGTGATGATAAACACGCTTTTGGGGTAATTGTGCCTGATGTGCCAGGTTGTTT
TTCTGCTGGAGATACATTAGAAGAGGCGTATATTAATGCAAAAGAAGCTATCGCTTTTCATATCGAGGGAATGTTAGAAG
ATGGTGAAGAGTTTCCTTTGCCAACCGATATAAAAGCACATATGAATAATAAAGAGTTTGAGGGGTTTACTTTTACTTTT
GTAGATGTTGATTTGTCGCATTTAATGGGTAAAGCGGAAAAAATTAATATTACTCTACCAGCGTTGTTATTACATAAAAT
TGATGCGTTTATTGCAACACATCCAGAGTATAAAAGCCGTAGTGGTTTTTTGGCACAACTTGCAACAGATAAATTACTTT
CAGCATAA

Structures

Antitoxin

• AlphaFold DB: A0A2S9ML45