Detailed information of TA system TA231861

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 1574270..1574925
• Replicon: chromosome
• Accession: NZ_CP090773
• Organism: Burkholderia multivorans strain 2007Ycf278

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: B9BGY2
• Locus tag: L0Z11_RS26445
• Protein ID: WP_006403072.1
• Coordinates: 1574270..1574689 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: L0Z11_RS26450
• Protein ID: WP_065496259.1
• Coordinates: 1574686..1574925 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0Z11_RS26420 (L0Z11_26420)	1569609..1570403	+	795	WP_105848189.1	AraC family transcriptional regulator	-
L0Z11_RS26425 (L0Z11_26425)	1570659..1571609	+	951	WP_217078394.1	DMT family transporter	-
L0Z11_RS26430 (L0Z11_26430)	1571805..1572380	+	576	WP_053298362.1	hypothetical protein	-
L0Z11_RS26435 (L0Z11_26435)	1572474..1572608	-	135	WP_006409923.1	entericidin A/B family lipoprotein	-
L0Z11_RS26440 (L0Z11_26440)	1572841..1574136	+	1296	WP_035487510.1	aspartate carbamoyltransferase	-
L0Z11_RS26445 (L0Z11_26445)	1574270..1574689	-	420	WP_006403072.1	type II toxin-antitoxin system VapC family toxin	Toxin
L0Z11_RS26450 (L0Z11_26450)	1574686..1574925	-	240	WP_065496259.1	Arc family DNA-binding protein	Antitoxin
L0Z11_RS26455 (L0Z11_26455)	1575230..1577476	+	2247	WP_006403070.1	TonB-dependent siderophore receptor	-
L0Z11_RS26460 (L0Z11_26460)	1577480..1578235	+	756	WP_006403069.1	tetratricopeptide repeat protein	-
L0Z11_RS26465 (L0Z11_26465)	1578232..1578915	+	684	WP_070554638.1	Fe2+-dependent dioxygenase	-
L0Z11_RS26470 (L0Z11_26470)	1578929..1579132	-	204	WP_006403067.1	hypothetical protein	-
L0Z11_RS26475 (L0Z11_26475)	1579721..1579864	+	144	WP_006409932.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-122)

Antitoxin

• Arc-like DNA binding domain (7-46)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 15086.28 Da
• Isoelectric Point: 5.2157

>T231861 WP_006403072.1 NZ_CP090773:c1574689-1574270 [Burkholderia multivorans]
MILVDTNVISEPLRREPSAAVIEWLDAQNVETLFLAAISLAEMRFGVAALPEGRRRDWLEQSIEHRVVPVFRGRILPFDD
AAGKAYARIRAHARAVGNAIAPADGYIAATAEANGLIVATRDVAPFEAAGLRVIDPWAS

Nucleotide

• Length: 420 bp

>T231861 NZ_CP126974:c4703162-4702878 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8750.96 Da
• Isoelectric Point: 5.1133

>AT231861 WP_065496259.1 NZ_CP090773:c1574925-1574686 [Burkholderia multivorans]
MPVITVRNLPDEVHRALRVRAALHGRSTEAEVRDILEQAVLSEGRVKLGTLLAEIGREAGGVDFDIQRDKTPTDPMSFE

Nucleotide

• Length: 240 bp

>AT231861 NZ_CP126974:c4703394-4703152 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCTGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• AlphaFold DB: B9BGY2