Detailed information of TA system TA023186

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 812953..813560
• Replicon: plasmid pRLG201
• Accession: NC_011368
• Organism: Rhizobium leguminosarum bv. trifolii WSM2304

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: B6A197
• Locus tag: RLEG2_RS25900
• Protein ID: WP_012556062.1
• Coordinates: 813306..813560 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: B6A196
• Locus tag: RLEG2_RS25895
• Protein ID: WP_003590983.1
• Coordinates: 812953..813309 (-)
• Length: 119 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RLEG2_RS35010	808541..808690	+	150	WP_012556058.1	hypothetical protein	-
RLEG2_RS25870	808797..809372	+	576	WP_012556059.1	alpha-ketoglutarate-dependent dioxygenase AlkB	-
RLEG2_RS25875	809411..809728	-	318	WP_012556060.1	hypothetical protein	-
RLEG2_RS25880	810049..811761	+	1713	WP_012556061.1	GAF domain-containing protein	-
RLEG2_RS25890	812155..812451	+	297	Protein_786	two pore domain potassium channel family protein	-
RLEG2_RS33655	812570..812724	-	155	Protein_787	SOS response-associated peptidase	-
RLEG2_RS25895	812953..813309	-	357	WP_003590983.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
RLEG2_RS25900	813306..813560	-	255	WP_012556062.1	type II toxin-antitoxin system HicA family toxin	Toxin
RLEG2_RS25905	813790..815133	-	1344	WP_012556063.1	PLP-dependent aminotransferase family protein	-
RLEG2_RS25910	815313..815504	+	192	WP_003590988.1	DUF1127 domain-containing protein	-
RLEG2_RS25915	815501..816280	+	780	WP_012556064.1	sulfite exporter TauE/SafE family protein	-
RLEG2_RS25920	816752..817393	-	642	WP_012556065.1	glyoxalase	-
RLEG2_RS25925	817374..818231	-	858	WP_003590993.1	SDR family oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..1266105	1266105

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (23-81)

Antitoxin

• Uncharacterised protein family HicB (54-104)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9336.75 Da
• Isoelectric Point: 10.2030

>T23186 WP_012556062.1 NC_011368:c813560-813306 [Rhizobium leguminosarum bv. trifolii WSM2304]
MNSKQRKTLAAIFKDPVSGTIEWTAIENLLVAAGADLIEGNGSRVKFEKDGIIASFHRPHPAKEAKRYQVRDAREFLMQI
GVTP

Nucleotide

• Length: 255 bp

>T23186 NC_011368:c813560-813306 [Rhizobium leguminosarum bv. trifolii WSM2304]
ATGAACAGCAAGCAGAGAAAGACTCTCGCGGCGATCTTCAAGGACCCGGTCTCGGGAACCATTGAATGGACCGCTATAGA
GAATCTGCTTGTGGCTGCCGGAGCCGACCTCATTGAGGGGAACGGTTCCCGCGTTAAGTTTGAAAAAGACGGGATCATTG
CGAGCTTCCATAGACCACATCCGGCCAAGGAAGCAAAGCGTTATCAGGTGCGCGATGCTCGCGAGTTCCTGATGCAGATT
GGAGTAACGCCATGA

Antitoxin

Protein

• Length: 119 a.a.
• Molecular weight: 13262.85 Da
• Isoelectric Point: 4.9838

>AT23186 WP_003590983.1 NC_011368:c813309-812953 [Rhizobium leguminosarum bv. trifolii WSM2304]
MNIMTYNGYHARIEFDAEDDVFFGRLAGISDVIGFHGDSVAELKKAFHEAVDDYLETCRKIGKEPQRPYSGKMMFRVAPE
VHRRAALAAELSGKSLNQWAEEVLEEAADHYAEARSPA

Nucleotide

• Length: 357 bp

>AT23186 NC_011368:c813309-812953 [Rhizobium leguminosarum bv. trifolii WSM2304]
ATGAACATCATGACCTACAACGGATATCACGCTCGCATCGAATTCGACGCTGAGGACGACGTGTTTTTCGGCAGGCTTGC
AGGTATCTCCGATGTAATCGGCTTTCACGGTGACAGTGTCGCTGAACTCAAGAAAGCCTTCCATGAGGCAGTCGATGACT
ACCTGGAGACCTGCAGGAAAATCGGCAAAGAGCCTCAGCGACCCTATTCTGGGAAGATGATGTTTCGTGTCGCTCCCGAA
GTCCATCGCCGAGCAGCTCTTGCTGCTGAGCTTTCGGGAAAGAGTCTGAACCAGTGGGCAGAAGAGGTGCTGGAAGAGGC
CGCCGACCATTATGCCGAGGCGCGCTCGCCGGCGTAG

Structures

Toxin

• AlphaFold DB: A0A7W5XSL5

Antitoxin

• AlphaFold DB: A0A7W9ZMG1