Detailed information of TA system TA231859

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 769039..769616
• Replicon: chromosome
• Accession: NZ_CP090773
• Organism: Burkholderia multivorans strain 2007Ycf278

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: L0Z11_RS22880
• Protein ID: WP_053298683.1
• Coordinates: 769039..769404 (-)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A2S9ML45
• Locus tag: L0Z11_RS22885
• Protein ID: WP_035956044.1
• Coordinates: 769398..769616 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0Z11_RS22855 (L0Z11_22855)	764216..765115	+	900	WP_035487807.1	lipid A hydroxylase LpxO	-
L0Z11_RS22860 (L0Z11_22860)	765239..765967	+	729	WP_249475708.1	hypothetical protein	-
L0Z11_RS22865 (L0Z11_22865)	766029..767627	-	1599	WP_053298685.1	alkyl hydroperoxide reductase subunit F	-
L0Z11_RS22870 (L0Z11_22870)	767746..768309	-	564	WP_006399806.1	alkyl hydroperoxide reductase subunit C	-
L0Z11_RS22875 (L0Z11_22875)	768755..768943	-	189	WP_249475710.1	hypothetical protein	-
L0Z11_RS22880 (L0Z11_22880)	769039..769404	-	366	WP_053298683.1	type II toxin-antitoxin system VapC family toxin	Toxin
L0Z11_RS22885 (L0Z11_22885)	769398..769616	-	219	WP_035956044.1	ribbon-helix-helix protein, CopG family	Antitoxin
L0Z11_RS22890 (L0Z11_22890)	769718..770614	-	897	WP_035954582.1	LysR substrate-binding domain-containing protein	-
L0Z11_RS22895 (L0Z11_22895)	770790..771950	+	1161	WP_249475712.1	branched-chain amino acid ABC transporter substrate-binding protein	-
L0Z11_RS22900 (L0Z11_22900)	772118..772657	+	540	WP_053298682.1	L-2-amino-thiazoline-4-carboxylic acid hydrolase	-
L0Z11_RS22905 (L0Z11_22905)	772650..773921	+	1272	WP_006403830.1	Zn-dependent hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-106)

Antitoxin

• Ribbon-helix-helix protein, CopG (6-40)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13556.55 Da
• Isoelectric Point: 5.1515

>T231859 WP_053298683.1 NZ_CP090773:c769404-769039 [Burkholderia multivorans]
MVKALFDTNILIDYLSGVESARLELGRYDHRAISTITWMEVLVGTTADDEGPIRAWLSSFDVIPLDSTVADRAVTIRKER
RIRLPDAIVWASAQVNGLLLVSRNTKDFPSTEPGIRVPYQI

Nucleotide

• Length: 366 bp

>T231859 NZ_CP126974:3983424-3983642 [Klebsiella pneumoniae subsp. pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8231.38 Da
• Isoelectric Point: 4.7640

>AT231859 WP_035956044.1 NZ_CP090773:c769616-769398 [Burkholderia multivorans]
MSRVLVDLSDGQLDELAVIVETEHRPRAAIIRDAIDAYIALHKRPLADDVFGLWKDRAVDGLAYQEKLRSEW

Nucleotide

• Length: 219 bp

>AT231859 NZ_CP126974:3983023-3983397 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A2S9ML45