Detailed information of TA system TA231854

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapC-vagC/VapC-VagC
• Location: 3294662..3295299
• Replicon: chromosome
• Accession: NZ_CP090770
• Organism: Burkholderia multivorans strain 2007Ycf875II

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A8E2UU98
• Locus tag: L0Z18_RS28270
• Protein ID: WP_035489546.1
• Coordinates: 3294662..3295063 (-)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: A0A132EWB1
• Locus tag: L0Z18_RS28275
• Protein ID: WP_006408845.1
• Coordinates: 3295063..3295299 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0Z18_RS28260 (L0Z18_28260)	3291983..3293125	+	1143	WP_053296686.1	alginate lyase family protein	-
L0Z18_RS28265 (L0Z18_28265)	3293152..3294540	+	1389	WP_006408285.1	L-serine ammonia-lyase	-
L0Z18_RS28270 (L0Z18_28270)	3294662..3295063	-	402	WP_035489546.1	type II toxin-antitoxin system VapC family toxin	Toxin
L0Z18_RS28275 (L0Z18_28275)	3295063..3295299	-	237	WP_006408845.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
L0Z18_RS28280 (L0Z18_28280)	3295487..3297190	+	1704	WP_006408280.1	thiamine pyrophosphate-binding protein	-
L0Z18_RS28285 (L0Z18_28285)	3297187..3298284	+	1098	WP_105761444.1	ADP-heptose--LPS heptosyltransferase	-
L0Z18_RS28290 (L0Z18_28290)	3298403..3299434	-	1032	WP_105761445.1	helix-turn-helix domain-containing protein	-
L0Z18_RS28295 (L0Z18_28295)	3299605..3300147	-	543	WP_006408276.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-121)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 15035.28 Da
• Isoelectric Point: 5.7429

>T231854 WP_035489546.1 NZ_CP090770:c3295063-3294662 [Burkholderia multivorans]
MPRYMLDTNMCIYLMKNQPEQVARRFAQCYTGDVVMSAITYAELEYGVTLCTNPARERRNLAALIEDIPVAPFDVAAAQA
YGPIREATRERKKDHLDKLIGAHAVSLDVVLVTNNERDFASYPGLRLENWLIG

Nucleotide

• Length: 402 bp

>T231854 NZ_CP126974:321827-322195 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8915.00 Da
• Isoelectric Point: 5.8651

>AT231854 WP_006408845.1 NZ_CP090770:c3295299-3295063 [Burkholderia multivorans]
MHTTRVFRNGNSQAVRIPADLAYERSDIELEIERVGDEIRIRPARRPLTGVLGKFAKFGPDFMAEGRGDQEQAEREGW

Nucleotide

• Length: 237 bp

>AT231854 NZ_CP126974:321609-321830 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A132EWB1